Osteopontin attenuation of dextran sulfate sodium-induced colitis in mice
Osteopontin attenuation of dextran sulfate sodium-induced colitis in mice
复制标题
DOI：
10.1038/labinvest.2009.80
复制
发表时间：
2009-10-01
影响因子：
5
通讯作者：
Sodek, Jaro
中科院分区：
文献类型：
--
作者：
da Silva, Andre Paes Batista;Ellen, Richard P.;Sodek, Jaro
骨桥蛋白（OPN）是一种存在于大多数组织和体液中的基质细胞因子；众所周知，它可以调节免疫反应。在先前使用葡聚糖硫酸钠（DSS）急性结肠炎模型的研究中，我们发现与野生型（WT）对照相比，OPN-null（(-/-)）小鼠的组织破坏加剧，修复减少。由于OPN通常存在于牛奶中，我们假设给药OPN可能保护肠道免受实验性结肠炎的不良影响。在DSS给药前24 h和给药期间分别给予20或2 μ g/ml溶解于饮用水中的牛乳OPN。结肠炎的临床参数和中性粒细胞功能分析与先前报道一致。口服给药的OPN在结肠黏膜中被吸收和免疫组化检测。20 μ g/ml的OPN-和dss处理的WT小鼠体重减轻37%，结肠缩短和脾脏增大减少(P
Osteopontin (OPN) is a matricellular cytokine present in most tissues and body fluids; it is known to modulate immune responses. In previous studies using the dextran sulfate sodium (DSS) acute colitis model, we found exacerbated tissue destruction and reduced repair in OPN-null ((-/-)) mice compared with wild-type (WT) controls. As OPN is normally present in milk, we hypothesized that administration of OPN may protect the intestines from the adverse effects of experimental colitis. A volume of 20 or 2 mu g/ml bovine milk OPN, dissolved in drinking water, was given to mice 24 h before, and during administration of DSS. Clinical parameters of colitis and neutrophil functions were analyzed as previously reported. Orally administered OPN was absorbed and detected in the colon mucosa by immunohistochemistry. The 20 mu g/ml OPN- and DSS-treated WT mice showed 37% less weight loss and reduced colon shortening and spleen enlargements than control mice (P