A transgenic apple callus showing reduced polyphenol oxidase activity and lower browning potential
A transgenic apple callus showing reduced polyphenol oxidase activity and lower browning potential
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DOI：
10.1271/bbb.65.383
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发表时间：
2001-02-01
影响因子：
1.6
通讯作者：
Itoh, Y
中科院分区：
文献类型：
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作者：
Murata, M;Nishimura, M;Itoh, Y
多酚氧化酶（PPO）负责苹果的酶促褐变。缺乏多酚氧化酶活性的苹果可能不仅对食品工业有用，而且对多酚代谢和多酚氧化酶功能的研究也有用。利用携带卡那霉素（KM）抗性基因和反义PPO基因的根杆菌制备了转基因苹果愈伤组织。从356个叶片外植体中获得4个抗km愈伤组织系。其中，3个转基因愈伤组织在含KM培养基上的生长速度与非转基因愈伤组织在不含KM培养基上的生长速度相同。其中1个愈伤组织含有反义PPO基因，其数量和活性降低到非转基因愈伤组织的一半。通过添加绿原酸估计，该品系的褐变电位也只有非转基因愈伤组织褐变电位的一半。
Polyphenol oxidase (PPO) is responsible for enzymatic browning of apples. Apples lacking PPO activity might be useful not only for the food industry hut also for studies of the metabolism of polyphenols and the function of PPO. Transgenic apple calli were prepared by using Argobacterium tumefaciens carrying the kanamycin (KM) resistant gene and antisense PPO gene. Four KM-resistant callus lines were obtained from 356 leaf explants. Among these transgenic calli, three calli grew on the medium containing KM at the same rate as non-transgenic callus on the medium without KM. One callus line had an antisense PPO gene, in which the amount and activity of PPO were reduced to half the amount and activity in non-transgenic callus. The browning potential of this line, which was estimated by adding chlorogenic acid, was also half the browning potential of non-transgenic callus.