DERMAL AND SEROLOGICAL RESPONSE AGAINST PSEUDOMONAS-AERUGINOSA IN SHEEP BRED FOR RESISTANCE AND SUSCEPTIBILITY TO FLEECE-ROT
DERMAL AND SEROLOGICAL RESPONSE AGAINST PSEUDOMONAS-AERUGINOSA IN SHEEP BRED FOR RESISTANCE AND SUSCEPTIBILITY TO FLEECE-ROT
复制标题
DOI：
10.1111/j.1751-0813.1991.tb09841.x
复制
发表时间：
1991-01-01
影响因子：
1.1
通讯作者：
WATTS, JE
中科院分区：
文献类型：
--
作者：
CHIN, JC;WATTS, JE
遗传选择的美利奴羊品系已在Trangie（新南威尔士州农业和渔业）培育，以获得对羊毛腐烂和蝇击的抗性（R）或易感性（S）。 据信，绒头性状是R或S表型的主要原因。 当转移到潮湿的沿海环境的悉尼，R和S羊不超过6周的羊毛覆盖，继续表现出显着差异的羊毛腐烂皮炎的发病率和严重程度。 为了检验这些绵羊的局部皮肤反应和免疫反应也可能存在差异的假设，每月一次皮内注射3次杀死的铜绿假单胞菌。 初次皮内攻击后，R羊皮肤硬结的发生率和炎症反应（硬结直径增加）均高于S羊。 与S羊相比，R羊也产生了更高水平的抗铜绿假单胞菌全细胞抗原和内外膜蛋白的循环抗体。 这些反应保持在R羊与每个连续的挑战，而S羊表现出下降的免疫反应。 在实验润湿条件下，通过铜绿假单胞菌的表皮攻击使来自每个遗传系的抗原性幼稚绵羊致敏时，还检测到针对外膜蛋白的抗体应答差异。 6个月后用外膜蛋白对这些动物进行皮内激发，发现R羊的硬结直径出现晚期最大值（72 h），而S羊的硬结直径在24 h时达到最大值。 但各组内24 h和72 h的硬结反应无显著差异。 这些差异伴随着显着更高的抗体滴度对外膜蛋白在R比S羊。 它的结论是，R和S绵羊在他们的皮肤和免疫反应不同，这种差异可能存在于皮肤的过程或响应环境抗原产生的机会和主要的羊毛腐烂易感皮肤细菌，铜绿假单胞菌。
Genetically select lines of Merino sheep have been bred at Trangie (NSW Agriculture and Fisheries) for resistance (R) or susceptibility (S) to fleece-rot and flystrike. It is believed that fleece characters are primarily responsible for the R or S phenotype. When transferred to the wetter coastal environment of Sydney, R and S sheep with no more than 6 weeks wool cover, continued to show significant differences in the incidence and severity of fleece-rot dermatitis. To test the hypothesis that these sheep might also exhibit differences in their local skin reactions and immune responsiveness, 3 intradermal injections of killed Pseudomonas aeruginosa were administered at monthly intervals. After primary intradermal challenge, R sheep had a higher incidence of skin induration and a stronger inflammatory response (increased induration diameter) than S sheep. Compared to S sheep, R sheep also developed higher levels of circulating antibodies against whole cell antigen and both inner and outer membrane proteins of P. aeruginosa. These responses were maintained in R sheep with each consecutive challenge while S sheep showed a decline in their immune responsiveness. Differences in antibody response against outer membrane proteins were also detected when antigenically naive sheep from each genetic line were sensitised by epicutaneous challenge with P. aeruginosa under experimental wetting conditions. Intradermal challenge of these animals 6 months later with outer membrane proteins, revealed a late maximum (72 h) in the development of induration diameters for R sheep while S animals showed maximal induration diameters by 24 h. However, there was no significant difference in induration response between 24 h and 72 h within each group of sheep. These differences were accompanied by significantly higher antibody titres against outer membrane proteins in R than S sheep. It is concluded that R and S sheep differ in their dermal and immune responsiveness, and that this difference may reside in the way the skin processes or responds to environmental antigens produced by the opportunistic and major fleece-rot predisposing skin bacteria, P. aeruginosa.