Monitoring temporary immunodepression by flow cytometric measurement of monocytic HLA-DR expression:: A multicenter standardized study
Monitoring temporary immunodepression by flow cytometric measurement of monocytic HLA-DR expression:: A multicenter standardized study
复制标题
DOI：
10.1373/clinchem.2005.052639
复制
发表时间：
2005-12-01
影响因子：
9.3
通讯作者：
Kunz, D
中科院分区：
文献类型：
--
作者：
Döcke, WD;Höflich, C;Kunz, D
背景：单中心试验表明，单核细胞 HLA-DR 是监测创伤、大手术或脓毒症后暂时性免疫抑制严重程度的良好标志物。现已推出用于测量单核细胞 HLA-DR 的新测试。 方法：我们在单实验室和实验室间实验中评估了用于单核细胞 HLA-DR 表达的新测试试剂组（BD Quantibrite (TM) HLA-DR/单核细胞试剂；Becton Dickinson），评估分析前处理、裂解不清洗 (LNW) 与裂解清洗 (LW) 值、参考值以及使用不同流量的效果结果：对于分析前处理、EDTA 抗凝、尽快在冰上储存以及采血后 4 小时内染色，所得结果与采集后立即分析的样品获得的值相当（单核细胞 HLA-DR 的平均增加类似于 4%）。 LNW 和 LW 的比较显示，LNW 的结果略高（与 LW 相比，LNW 高出 18%；r = 0.982）。不同流式细胞仪和仪器设置的比较给出了 CV
Background: Single-center trials have shown that monocytic HLA-DR is a good marker for monitoring the severity of temporary immunodepression after trauma, major surgery, or sepsis. A new test for measuring monocytic HLA-DR is now available.Methods: We evaluated a new test reagent set for monocytic HLA-DR expression (BD Quantibrite (TM) HLA-DR/Monocyte reagent; Becton Dickinson) in single-laboratory and interlaboratory experiments, assessing preanalytical handling, lyse-no-wash (LNW) vs lyse-wash (LW) values, reference values, and the effect of use of different flow cytometers and different instrument settings on test variance.Results: For preanalytical handling, EDTA anticoagulation, storage on ice as soon as possible, and staining within 4 h after blood collection gave results comparable to values obtained for samples analyzed immediately after collection (mean increase of similar to 4% in monocytic HLA-DR). Comparison of LNW and LW revealed slightly higher results for LNW (similar to 18% higher for LNW compared with LW; r = 0.982). Comparison of different flow cytometers and instrument settings gave CVs