Localization of Prohibitin in the Nuclear Matrix and Alteration of Its Expression During Differentiation of Human Neuroblastoma SK-N-SH Cells Induced by Retinoic Acid
Localization of Prohibitin in the Nuclear Matrix and Alteration of Its Expression During Differentiation of Human Neuroblastoma SK-N-SH Cells Induced by Retinoic Acid
复制标题
视黄酸诱导的人神经母细胞瘤 SK-N-SH 细胞分化过程中抑制素在核基质中的定位及其表达的改变。
DOI：
10.1007/s10571-010-9608-7
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发表时间：
2011-03-01
影响因子：
4
通讯作者：
Kong, Hai-Yan
中科院分区：
文献类型：
--
作者：
Li, Qi-Fu;Liang, Ying;Kong, Hai-Yan
选择性地提取维甲酸(RA)处理前后人神经母细胞瘤SK-N-SH细胞的核基质-中间丝系统，观察禁止素(PHB)在核基质中的分布及其与相关基因的共定位。双向凝胶电泳法(2-DE)、质谱仪(MS)鉴定和蛋白质免疫印迹分析结果均证实，PHB存在于SK-N-SH核基质蛋白组分中，RA治疗后PHB表达下调。免疫荧光显微镜观察显示，PHB定位于核基质中，其分布因RA治疗而改变。激光共聚焦显微镜结果显示，PHB与c-myc、c-fos、p53和Rb的表达产物共定位，但RA治疗后共定位区域发生改变。我们的结果证明PHB是一种核基质蛋白，定位于核基质纤维中。PHB在SK-N-SH细胞中的分布及其与相关原癌基因和抑癌基因的共存表明，PHB在SK-N-SH细胞的分化中起着关键作用，值得进一步研究。
The nuclear matrix-intermediate filament system of human neuroblastoma SK-N-SH cells before and after retinoic acid (RA) treatment was selectively extracted and the distribution of prohibitin (PHB) in the nuclear matrix, as well as its colocalization with related genes, was observed. Results of two-dimensional gel electrophoresis (2-DE), mass spectrometry (MS) identification, and protein immunoblotting all confirm that PHB was present in the components of SK-N-SH nuclear matrix proteins and was down-regulated after RA treatment. Immunofluorescence microscopy observations show that PHB was localized in the nuclear matrix and its distribution was altered due to RA treatment. Laser confocal microscopy results reveal that PHB colocalized with the expression products of c-myc, c-fos, p53, and Rb, but the colocalization region was altered after RA treatment. Our results prove that PHB is a nuclear matrix protein and is localized in nuclear matrix fibers. The distribution of PHB in SK-N-SH cells and its colocalization with related proto-oncogenes and tumor suppressor genes suggest that PHB plays pivotal roles in the differentiation of SK-N-SH cells and deserves further study.