UPARSE: highly accurate OTU sequences from microbial amplicon reads
UPARSE: highly accurate OTU sequences from microbial amplicon reads
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DOI：
10.1038/nmeth.2604
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发表时间：
2013-10-01
期刊：
影响因子：
48
通讯作者：
Edgar, Robert C.
中科院分区：
文献类型：
--
作者：
Edgar, Robert C.
扩增的标记基因序列可用于了解微生物群落结构，但它们受到高水平的测序和扩增伪影的影响。UPARSE管道报告了操作分类单位（OTU）序列，其中3%的错误碱基通常由其他方法报告。准确性的提高导致OTU的数量大大减少，始终更接近社区中预期的物种数量。
Amplified marker-gene sequences can be used to understand microbial community structure, but they suffer from a high level of sequencing and amplification artifacts. The UPARSE pipeline reports operational taxonomic unit (OTU) sequences with 3% incorrect bases commonly reported by other methods. The improved accuracy results in far fewer OTUs, consistently closer to the expected number of species in a community.