Microenvironmental Interaction Between Hypoxia and Endothelial Cells Controls the Migration Ability of Placenta-Derived Mesenchymal Stem Cells via α4 Integrin and Rho Signaling
Microenvironmental Interaction Between Hypoxia and Endothelial Cells Controls the Migration Ability of Placenta-Derived Mesenchymal Stem Cells via α4 Integrin and Rho Signaling
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DOI：
10.1002/jcb.25398
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发表时间：
2016-05-01
影响因子：
4
通讯作者：
Kim, Gi Jin
中科院分区：
文献类型：
--
作者：
Choi, Jong Ho;Lim, Seung Mook;Kim, Gi Jin
间充质干细胞（MSC）是退行性疾病细胞治疗的强大来源。间充质干细胞的迁移能力是细胞移植到靶组织或器官后增强治疗效果的重要因素。缺氧和内皮屏障是代表性的迁移微环境因素，已知在多种癌症中受到整合素介导的途径的调节。然而，它们在间充质干细胞中的调控机制仍不清楚。本研究的目的是比较依赖缺氧并与人脐静脉内皮细胞（HUVEC）共培养的胎盘源性间充质干细胞（PDMSC）中整合素介导的信号传导相关标志物的表达，并评估其迁移能力与包括缺氧和内皮细胞在内的微环境因素之间的相关性。与正常成纤维细胞（WI-38）和对照（P
Mesenchymal stem cells (MSCs) are a powerful source for cell therapy in degenerative diseases. The migration ability of MSCs is an important factor that enhances the therapeutic effect of the cells when they are transplanted into target tissues or organs. Hypoxia and the endothelial barrier, which are representative migration microenvironmental factors, are known to be regulated by the integrin-mediated pathway in several cancers. However, their regulatory mechanisms in MSCs remain unclear. Here, the objectives of the study were to compare the expression of markers related to integrin-mediated signaling in placenta-derived MSCs (PDMSCs) dependent on hypoxia and co-cultured with human umbilical vein endothelial cells (HUVECs) and to evaluate their correlations between migration ability and microenvironmetal factors including hypoxia and endothelial cells. The migration abilities of PDMSCs exposed to hypoxic conditions were significantly increased compared with normal fibroblasts (WI-38) and control (P