Modeling the PAO-GAO competition: Effects of carbon source, pH and temperature
Modeling the PAO-GAO competition: Effects of carbon source, pH and temperature
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DOI：
10.1016/j.watres.2008.10.032
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发表时间：
2009-02-01
期刊：
影响因子：
12.8
通讯作者：
van Loosdrecht, Mark C. M.
中科院分区：
文献类型：
--
作者：
Lopez-Vazquez, Carlos M.;Oehmen, Adrian;van Loosdrecht, Mark C. M.
利用包含碳源、温度和pH依赖关系的代谢模型，研究了不同碳源(醋酸丙酸比)、温度和pH水平对聚磷酸盐和糖原积累微生物(分别为PAO和GAO)之间竞争的影响。该模型令人满意地描述了在标准条件(20摄氏度和pH 7.0)下，在丙酸盐(HPR)和醋酸盐(HAC)上培养的PAO(Acumulibacter)和GAO(Composbacter and Alphaprobacteria-GAO)实验室浓缩培养物的细菌活性。利用校正后的模型，考察了不同进水HAC/HPR比例(100-0、75-25、50-50和0-100%)、温度(10、20和30℃)和pH水平(6.0、7.0和7.5)对不动杆菌、竞争杆菌和变形杆菌-GAO之间竞争的影响。主要目的是评估哪些条件有利于PAO的存在，从而有利于污水处理厂的生物除磷工艺。在低温(10℃)下，无论进水碳源或pH值如何，PAO都是优势微生物。在中等温度(20摄氏度)下，当同时供应HAc和HPR时，PAO占据主导地位(HAc和HPR的比例分别为75-25和50-50%)。然而，使用HAc或HPR作为20℃下的唯一碳源不利于PAO，除非使用高pH(7.5)。同时，在较高的温度(30℃)下，GAO往往是优势微生物。然而，在较高的污水温度(30摄氏度)下，进水中醋酸盐和丙酸的组合存在(75-25和50-50%HAc/HPR)以及较高的pH(7.5)似乎是有利于PAO比GAO代谢的潜在因素。(C)2008爱思唯尔有限公司。保留所有权利。
The influence of different carbon sources (acetate to propionate ratios), temperature and pH levels on the competition between polyphosphate- and glycogen-accumulating organisms (PAO and GAO, respectively) was evaluated using a metabolic model that incorporated the carbon source, temperature and pH dependences of these microorganisms. The model satisfactorily described the bacterial activity of PAO (Accumulibacter) and GAO (Competibacter and Alphaproteobacteria-GAO) laboratory-enriched cultures cultivated on propionate (HPr) and acetate (HAc) at standard conditions (20 degrees C and pH 7.0). Using the calibrated model, the effects of different influent HAc to HPr ratios (100-0, 75-25, 50-50 and 0-100%), temperatures (10, 20 and 30 degrees C) and pH levels (6.0, 7.0 and 7.5) on the competition among Accumulibacter, Competibacter and Alphoproteobacteria-GAO were evaluated. The main aim was to assess which conditions were favorable for the existence of PAO and, therefore, beneficial for the biological phosphorus removal process in sewage treatment plants. At low temperature (10 degrees C), PAO were the dominant microorganisms regardless of the used influent carbon source or pH. At moderate temperature (20 degrees C), PAO dominated the competition when HAc and HPr were simultaneously supplied (75-25 and 50-50% HAc to HPr ratios). However, the use of either HAc or HPr as sole carbon source at 20 degrees C was not favorable for PAO unless a high pH was used (7.5). Meanwhile, at higher temperature (30 degrees C), GAO tended to be the dominant microorganisms. Nevertheless, the combined presence of acetate and propionate in the influent (75-25 and 50-50% HAc to HPr ratios) as well as a high pH (7.5) appear to be potential factors to favor the metabolism of PAO over GAO at higher sewage temperature (30 degrees C). (C) 2008 Elsevier Ltd. All rights reserved.