Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
Supracellular Actomyosin Mediates Cell-Cell Communication and Shapes Collective Migratory Morphology
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细胞上肌动球蛋白介导细胞间通讯并塑造集体迁移形态
DOI：
10.1016/j.isci.2020.101204
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发表时间：
2020-06-26
期刊：
影响因子：
5.8
通讯作者：
Chen, Jiong
中科院分区：
文献类型：
--
作者：
Wang, Heng;Guo, Xuan;Chen, Jiong
在集体细胞迁移期间，前细胞倾向于延伸主要的前导突出，这很少存在于侧面或后位置的细胞中。我们使用果蝇边界细胞（BCS）作为集体迁移的模型系统，我们揭示了在BC簇的外围表面上存在上细胞肌球蛋白网络。我们证明了这种外周上超细胞网络所施加的肌球蛋白II介导的机械张力不仅介导了BC和非领导BC之间的介导的细胞 - 细胞通信，而且还限制了非领导BC的突出突起的形成。进一步的分析表明，取决于ARP2/3复合物功能的细胞质树突状肌动蛋白网络与Actomyosin网络相互作用。总之，我们的数据表明，由ARP2/3依赖性肌动蛋白聚合产生的向外推力或降突的力量以及由细胞上肌球蛋白网络产生的内向限制力共同决定了迁移BC的集体和极化形态。
During collective cell migration, front cells tend to extend a predominant leading protrusion, which is rarely present in cells at the side or rear positions. Using Drosophila border cells (BCs) as a model system of collective migration, we revealed the presence of a supracellular actomyosin network at the peripheral surface of BC clusters. We demonstrated that the Myosin II-mediated mechanical tension as exerted by this peripheral supracellular network not only mediated cell-cell communication between leading BC and non-leading BCs but also restrained formation of prominent protrusions at non-leading BCs. Further analysis revealed that a cytoplasmic dendritic actin network that depends on the function of Arp2/3 complex interacted with the actomyosin network. Together, our data suggest that the outward pushing or protrusive force as generated from Arp2/3-dependent actin polymerization and the inward restraining force as produced from the supracellular actomyosin network together determine the collective and polarized morphology of migratory BCs.