Heterogeneity of rat pituitary gonadotropins on electrofocusing; differences between sexes and after castration.
Heterogeneity of rat pituitary gonadotropins on electrofocusing; differences between sexes and after castration.
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电聚焦下大鼠垂体促性腺激素的异质性；
DOI：
10.1210/endo-111-2-385
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发表时间：
1982
期刊：
影响因子：
4.8
通讯作者：
S. Ellis
中科院分区：
文献类型：
--
作者：
D. Robertson;L. M. Foulds;S. Ellis
将完整的和去势后 4 周的雄性和雌性大鼠的垂体前叶提取物电聚焦在 pH 范围 3.5-10 的蔗糖密度梯度中。合并柱级分以覆盖0.5 pH单位的pH范围，并通过体外生物测定和RIA测定LH，并通过放射受体测定和RIA测定FSH。两性去势后，生物活性 LH 的 pH 分布都发生了改变，碱性 pH 范围内恢复活性的比例从完整动物的 52-57% 增加到去势后的 71-73%（P 小于 0.01）。此外，在 pH 值 7-9.5 范围内，雄性组 (37%) 比雌性组（发情期，30% 或发情前期，28%）恢复了显着更多的生物活性（P 小于 0.05）。 FSH受体结合活性位于pH值3.5-6.5范围内。在 pH 值范围 3.5-4.5 范围内，雌性组（分别为 39% 和 37% 的动情期和 37%）恢复的受体结合活性显着低于雄性组（61%；P 小于 0.05，P 小于 0.01）。免疫反应性 LH 和 FSH 的分布与 LH 体外生物测定和 FSH 放射受体测定观察到的分布相似。结论是，垂体促性腺激素的电荷分布根据动物的性别和去势后的变化而改变。这些发现进一步证明垂体产生的促性腺激素类型受到内分泌控制。
Anterior pituitary extracts from intact and 4 week postcastration male and female rats were electrofocused in sucrose density gradients within the pH range 3.5-10. Column fractions were combined to cover this pH range in 0.5 pH units and assayed for LH by in vitro bioassay and RIA and for FSH by radioreceptor assay and RIA. The pH distribution of bioactive LH was altered after castration in both sexes, with the proportion of recovered activity in the alkaline pH range increasing (P less than 0.01) from 52-57% in the intact animal to 71-73% after castration. In addition, significantly more bioactivity was recovered in the pH range 7-9.5 with the male (37%) than with either of the female (diestrous, 30% or proestrous, 28%) groups (P less than 0.05). FSH receptor binding activity was located in the pH region 3.5-6.5. Significantly less receptor binding activity was recovered in the pH range 3.5-4.5 with the female groups (39% and 37% diestrous and proestrous, respectively) than the male group (61%; P less than 0.05, P less than 0.01). The distribution of immunoreactive LH and FSH was similar to that observed with the LH in vitro bioassay and FSH radioreceptor assay. It is concluded that the charge distribution of pituitary gonadotropins is altered according to the sex of the animal and after castration. These findings provide further evidence that the type of gonadotropin produced by the pituitary is under endocrine control.