Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride
Antioxidant and proapoptotic effects of anthocyanins from bilberry extract in rats exposed to hepatotoxic effects of carbon tetrachloride
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DOI：
10.1016/j.lfs.2016.06.007
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发表时间：
2016-07-15
期刊：
影响因子：
6.1
通讯作者：
Sokolovic, Dusan
中科院分区：
文献类型：
--
作者：
Popovic, Dejan;Dukic, Davor;Sokolovic, Dusan
目的：本研究的目的是通过监测氧化应激和细胞凋亡的参数，并通过进行组织病理学和形态计量学分析来确定来自越橘提取物的花青素对暴露于四氯化碳（CCl 4）的大鼠的肝脏的保护作用。组I（0.9% NaCl-10天），第II组（越橘提取物，75 mg/kg-10天），第III组（0.9% NaCl-9天，第10天CCl 4 -2 ml/kg），组IV（越橘提取物，75毫克/公斤-10天，第十天CCl 4 -2毫升/公斤）。越桔提取物导致血清中生化指标活性显著降低与组III相比，组IV中的AST、GGT、LDH和ALT、促氧化酶黄嘌呤氧化酶的活性以及肝脏中的脂质过氧化水平（p < 0.01）。与第三组相比，第四组中越橘提取物导致抗氧化剂标记物-过氧化氢酶（p < 0.05）、超氧化物歧化酶、谷胱甘肽S-转移酶和谷胱甘肽过氧化物酶（p < 0.01）的活性以及还原型谷胱甘肽的浓度（p < 0.05）显著增加。越橘提取物的应用导致肝组织中凋亡肝细胞数量和caspase-3活性的增加（p < 0.01）。与第III组相比，第IV组中证实了凝固性坏死面积的减少（p <0.001）以及大泡肝细胞的数量（p <0.01），沿着有丝分裂活性的增加（p < 0.01）。意义：来自越橘提取物的花青素具有强抗氧化特性，因此可以被认为是天然产物中的强效保肝药。(C)2016 Elsevier Inc. All rights reserved.
Aims: The aim of this research was to determine the hepatoprotective effects of anthocyanins from bilberry extract in rats exposed to carbon tetrachloride (CCl4) by monitoring the parameters of oxidative stress and apoptosis, and by performing the histopathological and morphometric analyses.Main methods: Animals were divided into four groups: Group I (0.9% NaCl-10 days), Group II (bilberry extract, 75 mg/kg-10 days), Group III (0,9% NaCl-9 days, and on the tenth day CCl4-2 ml/kg), Group IV (bilberry extract, 75 mg/kg-10 days and on the tenth day CCl4-2 ml/kg).Key findings: Bilberry extract led to a significant decrease in the activity of biochemical parameters in serum(AST, GGT, LDH, and ALT), the activity of pro-oxidative enzyme xanthine oxidase, as well as the level of lipid peroxidation in the liver in Group IV compared to Group III (p < 0.01). Bilberry extract resulted in a significant increase in the activity of the antioxidant markers-catalase (p < 0.05), superoxide dismutase, glutathione S-transferase and glutathione peroxidase (p < 0.01), and the concentration of reduced glutathione (p < 0.05) in Group IV in relation to Group III. The application of bilberry extract resulted in an increase in the number of apoptotic hepatocytes and the activity of caspase-3 in the liver tissue (p < 0.01). The reduction of coagulation necrotic areas was proved (p < 0.001) as well as the number of macrovesicular hepatocytes (p < 0.01), along with an increased mitotic activity (p < 0.01) in Group IV compared to Group III.Significance: Anthocyanins from bilberry extract have strong antioxidant properties and therefore can be considered as powerful hepatoprotectives in natural products. (C) 2016 Elsevier Inc. All rights reserved.