Reversible and Irreversible Drought-Induced Changes in the Anther Proteome of Rice (Oryza sativa L.) Genotypes IR64 and Moroberekan
Reversible and Irreversible Drought-Induced Changes in the Anther Proteome of Rice (Oryza sativa L.) Genotypes IR64 and Moroberekan
复制标题
水稻 (Oryza sativa L.) 基因型 IR64 和 Moroberekan 干旱引起的可逆和不可逆的花药蛋白质组变化
DOI：
10.1093/mp/ssq039
复制
发表时间：
2011-01-01
期刊：
影响因子：
27.5
通讯作者：
Bennett, John
中科院分区：
文献类型：
--
作者：
Liu, Jian-Xiang;Bennett, John
作物产量对生殖生长期水分亏缺最为敏感。对水稻来说，最敏感的产量因素是小穗育性，最敏感的时期是抽穗前。在这里，我们研究了干旱对两种水稻基因型：Moroberekan和IR 64花药蛋白质组的影响。在抽穗前（3DBH），在充分浇水的对照中，停水3d，直到旗叶相对含水量（RWC）下降到45- 50%。然后再给植物浇水，2-3天后抽穗，相对于对照延迟4-5天。通过二维（2-D）凝胶电泳，在胁迫/复水植物和对照的3 DBH、胁迫期结束时和抽穗时分离了花药蛋白质，并且93个蛋白质点受到干旱的重复性影响。在实验期间，两种水稻基因型。干旱胁迫后，复水后，2种基因型中有24个蛋白点的表达不可逆，60个蛋白点在IR 64中不可逆，而在Moroberekan中可逆，9个蛋白点在Moroberekan中不可逆，而在IR 64中可逆。其中，有14个新的干旱诱导蛋白点在IR 64，没有一个是可逆的复水。然而，有13个新的干旱诱导的蛋白点在Moroberekan，其中10个是可逆的复水，包括6个干旱诱导的蛋白点，在IR 64中没有逆转。综上所述，我们的蛋白质组学数据表明，耐旱基因型Moroberekan具有更好的恢复能力，干旱和复水后，在花药蛋白质组水平比干旱敏感的基因型IR 64。本文还讨论了干旱对水稻花药发育和花粉细胞功能的影响。
Crop yield is most sensitive to water deficit during the reproductive stage. For rice, the most sensitive yield component is spikelet fertility and the most sensitive stage is immediately before heading. Here, we examined the effect of drought on the anther proteome of two rice genotypes: Moroberekan and IR64. Water was withheld for 3 d before heading (3DBH) in well watered controls for 5 d until the flag leaf relative water content (RWC) had declined to 45-50%. Plants were then re-watered and heading occurred 2-3 d later, representing a delay of 4-5 d relative to controls. The anther proteins were separated at 3 DBH, at the end of the stress period, and at heading in stressed/re-watered plants and controls by two-dimensional (2-D) gel electrophoresis, and 93 protein spots were affected reproducibly in abundance by drought during the experiment across two rice genotypes. After drought stress, upon re-watering, expressions of 24 protein spots were irreversible in both genotypes, 60 protein spots were irreversible in IR64 but reversible in Moroberekan, only nine protein spots were irreversible in Moroberekan while reversible in IR64. Among them, there were 14 newly drought-induced protein spots in IR64; none of them was reversible on re-watering. However, there were 13 newly drought-induced protein spots in Moroberekan, 10 of them were reversible on re-watering, including six drought-induced protein spots that were not reversed in IR64. Taken together, our proteomics data reveal that drought-tolerant genotype Moroberekan possessed better recovery capability following drought and re-watering at the anther proteome level than the drought-sensitive genotype IR64. The disruptions of drought to rice anther development and pollen cell functions are also discussed in the paper.