ANALYSIS OF AN ERWINIA-CHRYSANTHEMI GENE-CLUSTER INVOLVED IN PECTIN DEGRADATION
ANALYSIS OF AN ERWINIA-CHRYSANTHEMI GENE-CLUSTER INVOLVED IN PECTIN DEGRADATION
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DOI：
10.1111/j.1365-2958.1991.tb02149.x
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发表时间：
1991-09-01
影响因子：
3.6
通讯作者：
ROBERTBAUDOUY, J
中科院分区：
文献类型：
--
作者：
CONDEMINE, G;ROBERTBAUDOUY, J
菊欧文氏菌参与果胶降解的一组四个基因已得到表征。 这四个基因形成独立的转录单位，并受到负调控基因kdgR的调控。 其中两个基因的功能是已知的：kduD 编码 2-keto-3-脱氧葡萄糖酸氧化还原酶，kdul 编码 5-keto-4-脱氧糖醛酸异构酶，这是果胶降解途径的两种酶。 kdgC与周质家族的果胶酸裂合酶基因有36%的同源性，但其产物似乎不具有果胶分解活性。 第四个基因 kdgF 可能在菊花的致病性中发挥作用。 比较 kdgR 控制的所有基因的调控区域可以更好地定义 KdgR 结合位点共识。
A group of four genes of Erwinia chrysanthemi involved in pectin degradation has been characterized. These four genes form independent transcription units and are regulated by the negative regulatory gene, kdgR. The functions of two of these genes are known: kduD codes for the 2-keto-3-deoxygluconate oxydoreductase and kdul for the 5-keto-4-deoxyuronate isomerase, two enzymes of the pectin degradation pathway. kdgC has 36% homology with pectate lyase genes of the periplasmic family but its product does not seem to have pectinolytic activity. The fourth gene, kdgF, could have a role in the pathogenicity of E. chrysanthemi. A comparison of the regulatory regions of all the genes controlled by kdgR allowed better definition of the KdgR-binding-site consensus.