Oxygen diffusion through natural extracellular matrices: Implications for estimating "Critical thickness" values in tendon tissue engineering
Oxygen diffusion through natural extracellular matrices: Implications for estimating "Critical thickness" values in tendon tissue engineering
复制标题
DOI：
10.1089/tea.2006.0361
复制
发表时间：
2008-04-01
影响因子：
4.1
通讯作者：
Derwin, Kathleen A.
中科院分区：
文献类型：
--
作者：
Androjna, Caroline;Gatica, Jorge E.;Derwin, Kathleen A.
在三维组织工程中，氧是维持细胞增殖和活力以及细胞外基质（ECM）产生所必需的。通常，扩散是体外氧运输的主要方式；因此，确保充足的氧气供应是必不可少的。在这项研究中，我们确定了3种天然ecm的氧扩散系数，这些ecm正在被研究作为肌腱组织工程的构建支架：小肠粘膜下层（SIS）、人真皮（Alloderm (R)）和犬阔筋膜。采用标准扩散池系统测定扩散系数。每种基质类型的范围为：SIS: 7 × 10(-6) - 2 × 10(-5) cm(2)/s, Alloderm (R): 1.9 - 3.1 × 10(-5) cm(2)/s，犬阔筋膜：1.6 - 4 × 10(-5) cm(2)/s。我们在氧气通过细胞种子支架扩散的数学模型中使用这些天然ecm的实验氧扩散率数据来估计可在体外培养的细胞种子支架的临界尺寸。
Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (Alloderm (R)), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7 x 10(-6) - 2 x 10(-5) cm(2)/s, Alloderm (R): 1.9 - 3.1 x 10(-5) cm(2)/s, and canine fascia lata: 1.6 - 4 x 10(-5) cm(2)/s. We used the experimental oxygen diffusivity data for these natural ECMs in a mathematical model of oxygen diffusion through a cell-seeded scaffold to estimate the critical size of cell-seeded scaffold that can be cultured in vitro.