The root‐knot nematode effector MiPDI1 targets a stress‐associated protein, SAP, to establish disease in Solanaceae and Arabidopsis
The root‐knot nematode effector MiPDI1 targets a stress‐associated protein, SAP, to establish disease in Solanaceae and Arabidopsis
复制标题
根结线虫效应子 MiPDI1 以应激相关蛋白 SAP 为目标，在茄科和拟南芥中引发疾病
作者：
Zhao Jianlong;Joffrey Mejias;Michaël Quentin;Yongpan Chen;Janice de Almeida‐Engler;Zhenchuan Mao;Qinghua Sun;Qian Liu;Bingyan Xie;Pierre Abad;Bruno Favery;简恒
植物寄生线虫的食道腺分泌大量的效应物，但它们的分子作用方式在很大程度上仍然未知。我们鉴定了南方根结线虫二硫键异构酶（PDI）样效应蛋白（MiPDI1），原位杂交表明MiPDI1在南方根结线虫的亚腹面腺中特异性表达。在寄生阶段显著上调。免疫定位显示，在线虫迁移过程中，在植物的取食细胞内，MiPDI1分泌。宿主诱导的MiPDI1基因沉默影响了线虫感染宿主的能力，而MiPDI1的表达增加了对南方根结线虫的易感性，为MiPDI1在南方根结线虫寄生中的关键作用提供了证据。酵母双杂交、双分子荧光互补和免疫共沉淀试验表明，MiPDI1与番茄应激相关蛋白（SlSAP12）相互作用，该蛋白与氧化还原调节的AtSAP12相邻，我们的研究结果表明，在茄科和拟南芥中，MiPDI1通过调控SAP介导的氧化还原信号、防御和胁迫适应等途径，参与了植物对非生物胁迫和生物胁迫的应答。
Large amounts of effectors are secreted by the oesophageal glands of plant‐parasitic nematodes, but their molecular mode of action remains largely unknown. We characterized aMeloidogyne incognitaprotein disulphide isomerase (PDI)‐like effector protein (MiPDI1) that facilitates nematode parasitism.In situhybridization showed thatMiPDI1was expressed specifically in the subventral glands ofM.incognita. It was significantly upregulated during parasitic stages. Immunolocalization demonstrated MiPDI1 secretionin plantaduring nematode migration and within the feeding cells. Host‐induced silencing of theMiPDI1gene affected the ability of the nematode to infect the host, whereasMiPDI1expression inArabidopsisincreased susceptibility toM.incognita, providing evidence for a key role of MiPDI1 inM.incognitaparasitism.Yeast two‐hybrid, bimolecular fluorescence complementation and coimmunoprecipitation assays showed that MiPDI1 interacted with a tomato stress‐associated protein (SlSAP12) orthologous to the redox‐regulated AtSAP12, which plays an important role in plant responses to abiotic and biotic stresses.SAP12silencing or knocking out inNicotiana benthamianaand Arabidopsis increased susceptibility toM.incognita.Our results suggest that MiPDI1 acts as a pathogenicity factor promoting disease by fine‐tuning SAP‐mediated responses at the interface of redox signalling, defence and stress acclimation in Solanaceae andArabidopsis.