Yellow Fever Virus Reemergence and Spread in Southeast Brazil, 2016-2019
Yellow Fever Virus Reemergence and Spread in Southeast Brazil, 2016-2019
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DOI：
10.1128/jvi.01623-19
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发表时间：
2020-01-01
影响因子：
5.4
通讯作者：
Bispo de Filippis, Ana Maria
中科院分区：
文献类型：
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作者：
Giovanetti, Marta;Lima de Mendonca, Marcos Cesar;Bispo de Filippis, Ana Maria
黄热病病毒（YFV）最近在巴西重新出现，由于该病毒在东南部地区迅速传播，引起了严重关切。为了更好地了解巴西东南部最近爆发的YFV遗传多样性和动态，我们从圣埃斯皮里托和里约热内卢州的非人灵长类动物（NHP）和人类感染病例的流行曲线峰值产生了18个完整和几乎完整的基因组。18个YFV基因组的基因组测序揭示了在巴西有史以来最大的疫情之一期间黄热病病毒传播和传播的估计时间，来源和可能途径。我们发现，在最近的疫情期间，YFV在2016年至2019年期间多次从米纳斯吉拉斯重新引入圣埃斯皮里托和里约热内卢州。便携式测序数据的分析可以确定YFV的传播走廊。这些发现强化了这样一种观点，即持续的基因组监测策略可以提供有关病毒遗传多样性和传播动力学的信息，这可能有助于了解虫媒病毒的流行。重要性巴西的虫媒病毒感染，包括黄热病、登革热、寨卡和基孔肯雅热，导致相当高的发病率和死亡率，是紧迫的公共卫生问题。然而，我们对这些疫情的理解受到基因组数据有限的阻碍。在这项研究中，我们调查了YFV的遗传多样性和空间分布在当前的爆发，通过分析基因组数据，在巴西东南部地区没有被其他先前的研究。为了深入了解YFV的引入和传播途径，我们通过对来自东南地区的非人灵长类动物和感染患者的YFV基因组进行测序来追踪病毒。我们的研究提供了对YFV如何在巴西新地区启动传播的理解，并说明该领域的基因组学可以增强传染病监测和控制的传统方法。
The recent reemergence of yellow fever virus (YFV) in Brazil has raised serious concerns due to the rapid dissemination of the virus in the southeastern region. To better understand YFV genetic diversity and dynamics during the recent outbreak in southeastern Brazil, we generated 18 complete and nearly complete genomes from the peak of the epidemic curve from nonhuman primates (NHPs) and human infected cases across the Espirito Santo and Rio de Janeiro states. Genomic sequencing of 18 YFV genomes revealed the estimated timing, source, and likely routes of yellow fever virus transmission and dispersion during one of the largest outbreaks ever registered in Brazil. We showed that during the recent epidemic, YFV was reintroduced from Minas Gerais to the Espirito Santo and Rio de Janeiro states multiple times between 2016 and 2019. The analysis of data from portable sequencing could identify the corridor of spread of YFV. These findings reinforce the idea that continued genomic surveillance strategies can provide information on virus genetic diversity and transmission dynamics that might assist in understanding arbovirus epidemics.IMPORTANCE Arbovirus infections in Brazil, including yellow fever, dengue, zika, and chikungunya, result in considerable morbidity and mortality and are pressing public health concerns. However, our understanding of these outbreaks is hampered by the limited availability of genomic data. In this study, we investigated the genetic diversity and spatial distribution of YFV during the current outbreak by analyzing genomic data from areas in southeastern Brazil not covered by other previous studies. To gain insights into the routes of YFV introduction and dispersion, we tracked the virus by sequencing YFV genomes sampled from nonhuman primates and infected patients from the southeastern region. Our study provides an understanding of how YFV initiates transmission in new Brazilian regions and illustrates that genomics in the field can augment traditional approaches to infectious disease surveillance and control.