Formation of a multiple protein complex on the adenovirus packaging sequence by the IVa2 protein.
Formation of a multiple protein complex on the adenovirus packaging sequence by the IVa2 protein.
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IVa2 蛋白在腺病毒包装序列上形成多蛋白复合物。
DOI：
10.1128/jvi.02097-06
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发表时间：
2007
影响因子：
5.4
通讯作者：
Imperiale,MichaelJ
中科院分区：
文献类型：
--
作者：
Tyler,RyanE;Ewing,SeanG;Imperiale,MichaelJ
在腺病毒病毒粒子组装过程中，包装序列介导病毒基因组的衣壳化。该序列由七个功能单元组成，称为 A 重复序列。最近的证据表明，腺病毒 IVa2 蛋白结合包装序列并参与基因组的包装。 IVa2-包装序列相互作用的研究因难以纯化病毒感染细胞中产生的蛋白质或通过重组技术而受到阻碍。我们报告了腺病毒 IVa2 蛋白重组未标记版本的首次纯化，并在体外表征了其与包装序列的结合。我们的数据表明，包装序列中有多个 IVa2 结合位点，并且 IVa2 与 DNA 的结合需要 A 重复序列 5'-TTTG-(N8)-CG-3'。此外，我们提供了 IVa2 在包装序列上形成多聚体复合物的证据。这些数据支持一个模型，其中腺病毒 DNA 包装是通过在包装序列上形成 IVa2 多蛋白复合物来进行的。
During adenovirus virion assembly, the packaging sequence mediates the encapsidation of the viral genome. This sequence is composed of seven functional units, termed A repeats. Recent evidence suggests that the adenovirus IVa2 protein binds the packaging sequence and is involved in packaging of the genome. Study of the IVa2-packaging sequence interaction has been hindered by difficulty in purifying the protein produced in virus-infected cells or by recombinant techniques. We report the first purification of a recombinant untagged version of the adenovirus IVa2 protein and characterize its binding to the packaging sequence in vitro. Our data indicate that there is more than one IVa2 binding site within the packaging sequence and that IVa2 binding to DNA requires the A-repeat consensus, 5′-TTTG-(N8)-CG-3′. Furthermore, we present evidence that IVa2 forms a multimeric complex on the packaging sequence. These data support a model in which adenovirus DNA packaging occurs via the formation of a IVa2 multiprotein complex on the packaging sequence.