A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori
A conserved SUMOylation signaling for cell cycle control in a holocentric species Bombyx mori
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DOI：
10.1016/j.ibmb.2014.05.008
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发表时间：
2014-08-01
影响因子：
3.8
通讯作者：
Kusakabe, Takahiro
中科院分区：
文献类型：
--
作者：
Li, Zhiqing;Mon, Hiroaki;Kusakabe, Takahiro
SUMOylation 是一种重要的翻译后修饰，可调节包括细胞周期进程在内的多种细胞过程。尽管SUMO化途径已在许多真核生物中得到鉴定和研究，但SUMO化调节各种底物功能的机制仍知之甚少。在这里，我们利用模型物种，即拥有全着丝粒染色体的家蚕，来探索 SUMO 化系统在细胞周期调控中的作用。我们鉴定了家蚕基因组中参与 SUMOylation 途径的所有组件。我们的数据揭示了 SUMO 化基因的细胞周期依赖性转录、SUMO 化蛋白的定位以及培养的蚕细胞中 SUMO 化底物的丰度。重要的是，蚕细胞的增殖因干扰 SUMO 化基因表达而受到显着抑制，这可能是由于 SUMO 化缺陷细胞在 G2/M 期停滞所致。此外，SUMO化基因的破坏导致有丝分裂过程中全中心染色体的聚集和分离缺陷，这与该阶段SUMO化基因的高表达和整体SUMO化的高富集一致，表明家蚕的SUMO化系统对于细胞周期调节至关重要，在有丝分裂中具有特殊作用。 (C) 2014 Elsevier Ltd. 保留所有权利。
SUMOylation is an essential post-translational modification that regulates a variety of cellular processes including cell cycle progression. Although the SUMOylation pathway has been identified and investigated in many eukaryotes, the mechanisms of SUMOylation in regulating the functions of various substrates are still poorly understood. Here, we utilized a model species, the silkworm Bombyx mori that possesses holocentric chromosomes, to exploit the role of the SUMOylation system in cell cycle regulation. We identified all the components that are involved in the SUMOylation pathway in the silkworm genome. Our data revealed a cell cycle-dependent transcription of the SUMOylation genes, localization of the SUMOylation proteins, and abundance of the SUMOylation substrates in cultured silkworm cells. Importantly, the proliferation of the silkworm cells was strikingly inhibited by interference with SUMOylation genes expression, possibly due to an arrest of the SUMOylation-deficient cells at the G2/M phase. Furthermore, disruption of the SUMOylation genes induced the defects of holocentric chromosome congression and segregation during mitosis, which was consistent with high expressions of the SUMOylation genes and high enrichments of global SUMOylation at this stage, suggesting that the SUMOylation system in silkworm is essential for cell cycle regulation, with one particular role in mitosis. (C) 2014 Elsevier Ltd. All rights reserved.