Simultaneous Determination of Tetracyclines Residues in Bovine Milk Samples by Solid Phase Extraction and HPLC-FL Method
Simultaneous Determination of Tetracyclines Residues in Bovine Milk Samples by Solid Phase Extraction and HPLC-FL Method
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DOI：
10.5681/apb.2011.005
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发表时间：
2011-06-01
影响因子：
3.6
通讯作者：
Nemati, Mahboob
中科院分区：
文献类型：
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作者：
Abbasi, Mehran Mesgari;Babaei, Hossein;Nemati, Mahboob
简介：四环素（TCs）广泛应用于畜牧业，其在牛奶中的残留可能对人类造成有害影响本研究的目的是调查伊朗阿尔达比勒当地市场的各种牛奶样品中是否存在四环素类药物残留。方法：从阿尔达比勒市场采集了 114 个巴氏灭菌、灭菌和生奶样品。采用固相萃取法提取四环素、土霉素和金霉素（TCs）残留。 TCs残留量的测定采用高效液相色谱（HPLC）方法，使用荧光检测器。结果：所有样品（114个样品）中总TCs残留平均值为97.6±16.9ng/g，巴氏灭菌、灭菌和原料奶样品的总TCs残留平均值分别为87.1±17.7、112.0±57.3和154.0±66.3ng/g。所有样品中 25% 的点四，以及巴氏灭菌、灭菌和原料奶样品中分别有 24.4%、30% 和 28.6% 的 TC 残留量高于建议的最高水平（100 ng/g）。结论：本研究表明四环素残留量超过允许量。监管机构应确保动物挤奶前有适当的停药期，并对这些药物的使用进行明确的监督。
Introduction: Tetracyclines (TCs) are widely used in animal husbandry and their residues in milk may resultin harmful effects on human The aim of this study was to investigate the presence of TCs residues in various bovine milk samples from local markets of Ardabil, Iran. Methods: One hundred and fourteen pasteurized, sterilized and raw milk samples were collected from markets of Ardabil. Tetracycline, Oxytetracycline and Chlortetracycline (TCs) residues extraction carried out by Solid Phase Extraction method. Determination of TCs residues were performed by high performance liquid chromatography (HPLC) method using Fluorescence detector. Results: The mean of total TCs residues in all samples (114 samples) was 97.6 +/- 16.9ng/g and that of pasteurized, sterilized and raw milk samples were 87.1 +/- 17.7, 112.0 +/- 57.3 and 154.0 +/- 66.3ng/g respectively. Twenty five point four percent of the all samples, and24.4%, 30% and 28.6% of the pasteurized, sterilized and raw milk samples, respectively had higher TCs residues than the recommended maximum levels (100ng/g). Conclusion: This study indicates the presence of tetracycline residues more than allowed amount. Regulatory authorities should ensure proper withdrawal period before milking the animals and definite supervisions are necessary on application of these drugs.