Femtosecond laser-induced cell fusion
Femtosecond laser-induced cell fusion
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DOI：
10.1063/1.2890070
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发表时间：
2008-03
影响因子：
4
通讯作者：
Jixian Gong;Xue-Ming Zhao;Q. Xing;Fang Li;Huanyu Li;Yanfeng Li;L. Chai;Qing-yue Wang;A. Z
中科院分区：
文献类型：
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作者：
Jixian Gong;Xue-Ming Zhao;Q. Xing;Fang Li;Huanyu Li;Yanfeng Li;L. Chai;Qing-yue Wang;A. Z
用飞秒激光诱导细胞融合。用1.38×104W的飞秒激光脉冲照射两个接触的法夫酵母原生质体界面0.25s。约20min后，可见细胞融合，160min内两个细胞合并成一个较大的细胞。在此功率水平下，融合率为80%。实验表明，飞秒激光诱导细胞融合存在阈值功率。以飞秒激光脉冲为触发，提出了飞秒激光诱导细胞融合的假设模型。
Femtosecond laser was employed to induce cell fusion. The interface between two protoplasts of Phaffia rhodozyma in contact was irradiated by femtosecond laser pulses with 1.38×104W for 0.25s. After about 20min, the fusion of cells became visible and finally two cells merged into one larger cell within 160min. Fusion rate at this power level was 80%. The experiments suggested the existence of threshold power for cell fusion induced by femtosecond laser. Regarding femtosecond laser pulses as a trigger, we present a hypothetical model for femtosecond laser-induced cell fusion.