How, When, and Where New Inhibitory Neurons Release Neurotransmitters in the Adult Olfactory Bulb
How, When, and Where New Inhibitory Neurons Release Neurotransmitters in the Adult Olfactory Bulb
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DOI：
10.1523/jneurosci.4543-10.2010
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发表时间：
2010-12-15
影响因子：
5.3
通讯作者：
Lledo, Pierre-Marie
中科院分区：
文献类型：
--
作者：
Bardy, Cedric;Alonso, Mariana;Lledo, Pierre-Marie
成年人出生的神经元不断地整合到嗅球中，在那里它们迅速地与各种突触输入建立联系。然而，很少有人知道他们的输出的功能特性。表征新神经元的突触输出对于评估成年神经发生对成熟回路的功能影响至关重要。在这里，我们使用光遗传学来控制新神经元的神经递质释放。我们发现，光诱导的突触GABA释放从成人出生的神经元导致突触后靶放电模式的深刻修改。我们发现，功能性输出突触在新细胞获得发放能力后立即形成，但大多数突触是在一个月后形成的。尽管新突触募集的时间存在差异，但突触后信号的性质保持不变。值得注意的是，我们发现嗅球回路的所有主要细胞类型，包括输出神经元和几种不同的局部中间神经元亚型，都与成人出生的神经元接触。因此，这项研究为新神经元如何整合到成人神经网络中并可能影响嗅觉提供了新的见解。
Adult-born neurons continuously incorporate into the olfactory bulb where they rapidly establish contacts with a variety of synaptic inputs. Little is known, however, about the functional properties of their output. Characterization of synaptic outputs from new neurons is essential to assess the functional impact of adult neurogenesis on mature circuits. Here, we used optogenetics to control neurotransmitter release from new neurons. We found that light-induced synaptic GABA release from adult-born neurons leads to profound modifications of postsynaptic target firing patterns. We revealed that functional output synapses form just after new cells acquire the faculty to spike, but most synapses were made a month later. Despite discrepancies in the timing of new synapse recruitment, the properties of postsynaptic signals remain constant. Remarkably, we found that all major cell types of the olfactory bulb circuit, including output neurons and several distinct subtypes of local interneurons, were contacted by adult-born neurons. Thus, this study provides new insights into how new neurons integrate into the adult neural network and may influence the sense of smell.