The enucleation of cells on plastic Leighton coverslips.
The enucleation of cells on plastic Leighton coverslips.
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塑料 Leighton 盖玻片上的细胞去核。
DOI：
10.3109/10520298109067291
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Kuhn
中科院分区：
文献类型：
--
作者：
N. Bols;K. Kuhn
描述了一种简单的细胞去核技术，便于放射自显影和电子显微镜检查的细胞色素。细胞在市售塑料Leighton盖玻片上生长，并在细胞松弛素B存在下离心。离心分离不需要特殊的支架，只需要高速离心机。中国仓鼠成纤维细胞和小鼠B-82细胞的去核率大于90%。
A simple enucleation technique that facilitates autoradiographic and electron microscopic examination of cytoplasms is described. Cells were grown on commercially available plastic Leighton coverslips and these were centrifuged in the presence of cytochalasin B. The centrifugation requires no special holders and only a high speed centrifuge. Enucleation frequencies of greater than 90% were obtained for Chinese hamster fibroblasts and mouse B-82 cells.