Molecular developmental mechanism in polypterid fish provides insight into the origin of vertebrate lungs.
Molecular developmental mechanism in polypterid fish provides insight into the origin of vertebrate lungs.
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DOI：
10.1038/srep30580
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发表时间：
2016-07-28
影响因子：
4.6
通讯作者：
Okabe M
中科院分区：
文献类型：
--
作者：
Tatsumi N;Kobayashi R;Yano T;Noda M;Fujimura K;Okada N;Okabe M
肺是四足动物呼吸空气的重要器官，起源于脊椎动物陆系化之前。因此，为了更好地了解肺的进化，我们研究了现存的基生放线翅目鱼类塞内加尔Bichir(Polypterus Senegalus)的肺发育。首先，我们在组织学上证实，该物种的肺发育与四足动物非常相似。我们还发现，已知在四足动物早期肺发育中起重要作用的三个基因(Fgf10、Tbx4和Tbx5)的间质表达模式与四足动物非常相似。此外，我们在Bichir和腔棘鱼的基因组中发现了Tbx4核心肺间充质特异性增强子(C-LME)，并实验证实这些增强子在四足动物中具有功能。这些发现提供了第一个分子证据，表明肺的发育程序已经在放线蕨类和石棺蕨类的共同祖先中建立。
The lung is an important organ for air breathing in tetrapods and originated well before the terrestrialization of vertebrates. Therefore, to better understand lung evolution, we investigated lung development in the extant basal actinopterygian fish Senegal bichir (Polypterus senegalus). First, we histologically confirmed that lung development in this species is very similar to that of tetrapods. We also found that the mesenchymal expression patterns of three genes that are known to play important roles in early lung development in tetrapods (Fgf10, Tbx4, and Tbx5) were quite similar to those of tetrapods. Moreover, we found a Tbx4 core lung mesenchyme-specific enhancer (C-LME) in the genomes of bichir and coelacanth (Latimeria chalumnae) and experimentally confirmed that these were functional in tetrapods. These findings provide the first molecular evidence that the developmental program for lung was already established in the common ancestor of actinopterygians and sarcopterygians.