XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE
XYLITOL PRODUCTION BY RECOMBINANT SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1038/nbt1191-1090
复制
发表时间：
1991-11-01
期刊：
影响因子：
--
通讯作者：
KERANEN, S
中科院分区：
文献类型：
--
作者：
HALLBORN, J;WALFRIDSSON, M;KERANEN, S
用树干毕赤酵母CBS 6054的木糖还原酶(XR)基因转化酿酒酵母，获得了高效转化木糖为木糖醇的方法。XYL1基因的染色体拷贝和cDNA拷贝的比较表明，基因组XYL1不含内含子，XR单体编码318个氨基酸(35,985D)，编码一个954个核苷酸的开放阅读框。该菌的氨基酸序列与多种醛糖还原酶相似，提示该菌属于醛酮还原酶超家族的一部分。转化XYL1基因的酿酒酵母可将木糖转化为木糖醇，转化率可达95%以上，这是天然木糖利用酵母所不能达到的。
We obtained efficient conversion of xylose to xylitol by transforming Saccharomyces cerevisiae with the gene encoding the xylose reductase (XR) of Pichia stipitis CBS 6054. Comparison of the chromosomal and cDNA copies of the XYL1 gene showed that the genomic XYL1 contains no introns, and an XR monomer of 318 amino acids (35,985 D) is encoded by an open reading frame of 954 bp. The amino acid sequence of the P. stipitis XR is similar to several aldose reductases, suggesting that P. stipitis XR is part of the aldoketo reductase superfamily. S. cerevisiae transformed with the XYL1 gene gave over 95% conversion of xylose into xylitol, a yield not obtainable with natural xylose utilizing yeasts.