Estrogen affects the cellular metabolism of the anterior cruciate ligament - A potential explanation for female athletic injury
Estrogen affects the cellular metabolism of the anterior cruciate ligament - A potential explanation for female athletic injury
复制标题
DOI：
10.1177/036354659702500521
复制
发表时间：
1997-09-01
影响因子：
4.8
通讯作者：
Lane, JM
中科院分区：
文献类型：
--
作者：
Liu, SH;AlShaikh, RA;Lane, JM
该实验室的研究已经证实了人类前交叉韧带中存在雌激素受体。本研究进一步研究了17 β-雌二醇对兔前交叉韧带成纤维细胞增殖和胶原合成的影响，分别通过测量[H-3]胸苷和[C-14]羟脯氨酸掺入来评估（0.0029、0.025、0.25、2.5和25 ng/ml）。随着雌二醇浓度的增加，胶原合成明显减少（P < 0.001）。在17 β-雌二醇浓度为0.025 ng/ml时首次观察到胶原蛋白合成下降。在正常生理水平的雌激素（0.025至0.25 ng/ml），胶原蛋白的合成减少了超过40%的控制，并在药理水平的2.5和25 ng/ml，超过50%的控制。随着雌二醇浓度的增加，也观察到成纤维细胞增殖显著减少（P = 0.023）。临床上，雌激素波动引起的前交叉韧带细胞代谢的改变可能会改变韧带的组成，使其更容易受伤。
Investigations from this laboratory have established the presence of estrogen receptors in the human anterior cruciate ligament. This study further investigates the effects of 17 beta-estradiol on the cellular proliferation and collagen synthesis of fibroblasts derived from the rabbit anterior cruciate ligament, Fibroblast proliferation and collagen synthesis in response to near log concentrations of 17 beta-estradiol (at 0.0029, 0.025, 0.25, 2.5, and 25 ng/ml) were assessed by measuring [H-3]thymidine and [C-14]hydroxyproline incorporation, respectively. Collagen synthesis was significantly reduced with increasing local estradiol concentration (P < 0.001). Declining collagen synthesis was first noted at a 17 beta-estradiol concentration of 0.025 ng/ml. Within normal physiologic levels of estrogen (0.025 to 0.25 ng/ml), collagen synthesis was reduced by more than 40% of control, and at pharmacologic levels of 2.5 and 25 ng/ml, by more than 50% of control. A significant reduction of fibroblast proliferation was also observed with increasing estradiol concentrations (P = 0.023). Clinically, alterations in anterior cruciate ligament cellular metabolism caused by estrogen fluctuations may change the composition of the ligament, rendering it more susceptible to injury.