Analysis of Grapevine’s Somatic Embryogenesis Receptor Kinase (SERK) Gene Family: VqSERK3/BAK1 Overexpression Enhances Disease Resistance
Analysis of Grapevine’s Somatic Embryogenesis Receptor Kinase (SERK) Gene Family: VqSERK3/BAK1 Overexpression Enhances Disease Resistance
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葡萄树体细胞胚胎发生受体激酶 (SERK) 基因家族分析：VqSERK3/BAK1 过表达增强抗病性
DOI：
10.1094/phyto-04-21-0136-r
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Xiping Wang
中科院分区：
文献类型：
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作者：
Yajuan Li;Chen Jiao;Zhenjiang Wei;Shengyue Chai;Hui Jia;Min Gao;Jessica Allison;Zhi Li;Chang-bing Song;Xiping Wang
体细胞胚胎发生受体激酶(SERK)基因家族在多种植物中得到了广泛的研究。本研究证实了中国野生葡萄中存在5个SERK基因，分别命名为VqSERK1、VqSERK2、VqSERK3、VqSERK4和VqSERK5。对这些Serk蛋白的预测结构分析表明，它们包括一个信号肽结构域、一个亮氨酸拉链结构域、一个Ser-Pro-Pro结构域、一个单一的跨膜结构域、不同的富含亮氨酸的重复序列和一个胞内激酶活性结构域。在白粉病和激素(水杨酸、茉莉酸、脱落酸和乙烯)处理下，葡萄的SERK基因表现出不同的基因表达模式。亚细胞定位分析证实VqSERK家族蛋白定位于细胞膜。此外，我们还从中国野生葡萄品种‘尚-24’中克隆了SERK3/BAK1基因。异源VqSERK3/BAK1在拟南芥bak1-4突变体中的表达恢复了对细胞死亡的控制，提高了对白粉病的抗性，并增强了气孔免疫。我们的工作为进一步研究葡萄抗病原菌和激素处理中的SERK基因奠定了基础。
The somatic embryogenesis receptor kinase (SERK) gene family has been intensively studied in several plant species. Here we confirmed the existence of five SERK genes in grapevine (Chinese wild grapevine Vitis quinquangularis) and named them VqSERK1, VqSERK2, VqSERK3, VqSERK4, and VqSERK5. Analysis of the predicted structures of these SERK proteins revealed they include a signal peptide domain, a leucine zipper domain, a Ser-Pro-Pro domain, a single transmembrane domain, different leucine-rich repeats, and an intracellular kinase activity domain. The SERK genes of grapevine showed different gene expression patterns when treated with powdery mildew (Erysiphe necator) and hormones (salicylic acid, jasmonic acid, abscisic acid, and ethylene). Subcellular localization assays confirmed that VqSERK family proteins localized to the cell membrane. Moreover, we cloned the SERK3/BAK1 gene from the Chinese wild grapevine V. quinquangularis clone ‘Shang-24’. Heterologous VqSERK3/BAK1 expression in the Arabidopsis bak1-4 mutant lines restored control of cell death, increased resistance to powdery mildew, and strengthened stomatal immunity. Our work may provide the foundation for further studies of SERK genes for pathogen resistance and hormone treatment in grapevine.