Comparing the effects of GA-responsive dwarfing genes Rht13 and Rht8 on plant height and some agronomic traits in common wheat
Comparing the effects of GA-responsive dwarfing genes Rht13 and Rht8 on plant height and some agronomic traits in common wheat
复制标题
DOI：
10.1016/j.fcr.2015.04.010
复制
发表时间：
2015-08-01
影响因子：
5.8
通讯作者：
Hu, Yin-Gang
中科院分区：
文献类型：
--
作者：
Wang, Yushen;Du, Yingying;Hu, Yin-Gang
Rht8 是唯一用于雨养或灌溉有限地区种植的小麦的 GA 响应矮化基因。为了评估替代矮化基因，本研究将 GA 响应矮化基因 Rht13 对株高和一些农艺性状的影响与 Rht8 进行了比较。使用源自 Jinmai47 (Rht8) 和 Magnif M1 (Rht13) 杂交的 F-2 个体、F-2:3 和 F-3:4 系的四个纯合基因型来评估和比较 Rht13 和 Rht8 的效果。胚芽鞘长度和幼苗根系特征是在实验室条件下进行的，而株高和各种产量组成部分是在中国西北地区的田间环境中进行评估的。 Rht13显着缩短了所有节间（除基部节间）的长度，最终株高平均降低了30.3%（30.1 cm），而Rht8主要仅降低了花序梗长度，使株高仅缩短了15.7%（16.0 cm）。 Rht13对株高的影响较大，导致穗至旗叶叶舌的距离减少了97.7%。 Rht13 与 Rht8 结合时的效果甚至更大 (104.9%)，导致花穗未能完全从旗叶鞘中露出。与高基因型相比，Rht13或Rht8对胚芽鞘长度和幼苗根部特征的影响均不显着，这可能有利于在雨养和灌溉受限地区利用Rht13或Rht13 + Rht8。在单独使用Rht13的品系（18.2%）和同时使用两个矮化基因（Rht13 + Rht8，18.9%）的品系中观察到较少的谷粒穗（-1），而单独使用Rht8的品系和高株系之间没有显着差异。 Rht13和Rht8分别使植物(-1)生物量减少27.9%和25.2%，使植物(-1)产量分别减少29.0%和17.4%。 Rht13 不影响千粒重和收获指数，而 Rht8 显着增加千粒重和收获指数。在西北地区雨养冬小麦环境中，Rht13显着降低了穗粒数(-1)。这种效应可能是由于从穗到旗叶叶舌的距离非常短。这种不良影响可以通过选择来改善。如果是这样，那么在雨养和灌溉有限的地区使用 Rht13 来改良小麦就有潜力。 (C) 2015 Elsevier B.V. 保留所有权利。
Rht8 is the only GA-responsive dwarfing gene used in wheat grown in rainfed or irrigation-limited areas. To assess an alternative dwarfing gene, the effects of GA-responsive dwarfing gene Rht13 on plant height and some agronomic traits were compared with Rht8 in this study. The four homozygous genotypes of the F-2 individuals, F-2:3 and F-3:4 lines derived from the cross between Jinmai47 (Rht8) and Magnif M1 (Rht13) were used to evaluate and compare the effects of Rht13 and Rht8. The coleoptile length and seedling root characteristics was conducted under laboratory conditions, while plant height and various yield components were evaluated in field environments in northwest China. Rht13 significantly shortened the length of all internodes (except the basal internode) and reduced final plant height by 30.3% (30.1 cm) on average, while Rht8 mainly reduced peduncle length alone to obtain a plant stature shortened by only 15.7% (16.0 cm). The greater effect of Rht13 on plant height resulted in the distance from spike to flag leaf ligule being decreased by 97.7%. The effect of Rht13 was even greater when combined with Rht8 (104.9%), such that the spikes failed to fully emerge from the flag leaf sheath. Compared with tall genotypes, coleoptile length and seedling root characteristics were not significantly affected by either Rht13 or Rht8, which might favor the utilization of Rht13 or Rht13 + Rht8 in rainfed and irrigation-limited areas. Fewer grains spike(-1) were observed in lines with Rht13 alone (18.2%) and with both dwarfing genes (Rht13 + Rht8, 18.9%), whereas there was no significant difference between the lines with Rht8 alone and tall lines. Rht13 and Rht8 reduced biomass plant(-1) by 27.9% and 25.2% and grain yield plant(-1) by 29.0% and 17.4%, respectively. Rht13 did not affect 1000-kernel weight and harvest index whereas Rht8 significantly increased 1000-kernel weight and harvest index. In the rainfed winter wheat environment encountered in northwest China, Rht13 reduced grain number spike(-1) significantly. This effect may be due to the very short distance from spike to flag leaf ligule. This undesirable effect may be improved through selection. If so, then there is potential for using Rht13 in wheat improvement in rainfed and irrigation-limited areas. (C) 2015 Elsevier B.V. All rights reserved.