Genetic modification tools in mosquitoes revolutionize our ability to functionally analyze insecticide resistance Genetic modification tools in mosquitoes revolutionize our ability to functionally analyze insecticide resistance
Genetic modification tools in mosquitoes revolutionize our ability to functionally analyze insecticide resistance Genetic modification tools in mosquitoes revolutionize our ability to functionally analyze insecticide resistance
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蚊子基因改造工具彻底改变了我们功能分析杀虫剂抗性的能力 蚊子基因改造工具彻底改变了我们功能分析杀虫剂抗性的能力
DOI：
10.1127/entomologia/2023/1897
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发表时间：
2023
影响因子：
6.9
通讯作者：
Grigoraki L
中科院分区：
文献类型：
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作者：
Grigoraki L
蚊子传播的疾病具有全球重要性，在全世界造成发病率和死亡率。预防这些疾病的主要方法是通过病媒控制。以杀虫剂为基础的病媒控制干预措施取得了令人印象深刻的成果，但现在受到蚊子种群中杀虫剂抗药性水平不断上升的威胁。分子和生物化学研究揭示了与杀虫剂抗性表型相关的几种机制，这是设计缓解策略的重要第一步。在某些情况下，已经实现了机制的功能验证，然而，许多候选基因和遗传修饰的作用（如果有的话）仍然难以捉摸。我们还缺乏对每种机制的效应大小以及不同机制如何相互作用以确定表型强度的关键知识。在这篇综述中，我们重点介绍了蚊子中可用的遗传修饰工具，以及它们的使用如何帮助我们更好地了解杀虫剂抗性的机制。我们还介绍了这些工具已被用于验证解毒酶和靶位点突变的作用，并讨论了这种方法的优点和局限性相比，其他可用的方法的研究。
Mosquito transmitted diseases are of global importance causing morbidity and mortality worldwide. The main way to prevent these diseases is through vector control. Insecticide-based vector control interventions have had impressive results, but are now threatened by the rising levels of insecticide resistance in mosquito populations. Molecular and biochemical research has revealed several mechanisms associated with the phenotype of insecticide resistance, which is an important first step for designing mitigation strategies. Functional validation of the mechanisms has been achieved in some cases, however, the role (if any) of many candidate genes and genetic modifications remains elusive. We also lack critical knowledge on the effect size of each mechanism and how the different mechanisms interact to determine the intensity of the phenotype. In this review we focus on the available genetic modification tools in mosquitoes and how their use can help us better understand the mechanisms of insecticide resistance. We also present studies where these tools have been used to validate the role of detoxification enzymes and target site mutations and discuss the advantages and limitations of this approach compared to other available methodologies.