Identification and evaluation of antioxidant activities of bamboo extracts.
Identification and evaluation of antioxidant activities of bamboo extracts.
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DOI：
10.1007/s11632-004-0001-9
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Li, J. Z.
中科院分区：
文献类型：
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作者：
Jun, M.;Tohru, U.;Li, J. Z.
以1，1-二苯基-2-苦基肼（DPPH）自由基清除率、亚油酸过氧化抑制率和还原能力为指标，研究了日本毛竹（Phyllostachys pubescens）和竹竹（P. bakeoides）的抗氧化活性。毛竹秆和麻达克竹叶的甲醇提取物具有较强的抗氧化活性，而清除DPPH自由基的活性较强。用不同溶剂对毛竹甲醇提取物进行了进一步的分级，正丁醇萃取物清除DPPH自由基的活性最强。正丁醇萃取物采用氯仿-甲醇梯度洗脱，硅胶柱层析分离。通过清除稳定的DPPH自由基来测量分离的级分的抗氧化活性。观察到大多数洗脱组分均具有抗氧化活性。氯仿-甲醇（10：1-5：1）洗脱得到的组分抗氧化活性较强。
The antioxidant activity of solvent extracts from two main bamboo species, moso bamboo (Phyllostachys pubescens) and madake bamboo (P. bambusoides) in Japan, was first evaluated by scavenging free radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH), the inhibition activity for peroxidation of linoleic acid, and the reduction power. The methanol-extracts of moso bamboo culms and madake bamboo leaves presented stronger antioxidant activity compared with DPPH scavenging activity. Methanol-extract of moso bamboo culms was further fractionated by different solvents and n-butanol soluble fraction exhibited the most significant activity in the DPPH scavenging assay. The fractionation of n-butanol soluble extract was isolated by silica gel column with gradient mixture solvent of chloroform and methanol. The isolated fractions were directed by the antioxidant activity measured by scavenging the stable DPPH free radical. It was observed that most of the eluted fractions showed the antioxidative activity. Fractions acquired from elution with the mixture solvent of chloroform and methanol (10:1-5:1) showed stronger antioxidant activity than the other fractions.