Production of testosterone, 5 alpha-androstane-3 alpha, 17 beta-diol and androsterone by dispersed testicular interstitial cells and whole testes in vitro.
Production of testosterone, 5 alpha-androstane-3 alpha, 17 beta-diol and androsterone by dispersed testicular interstitial cells and whole testes in vitro.
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分散的睾丸间质细胞和整个睾丸在体外产生睾酮、5α-雄甾烷-3α、17β-二醇和雄酮。
DOI：
10.1677/joe.0.0800321
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
W. H. Moger
中科院分区：
文献类型：
--
作者：
W. H. Moger
在体外研究了用胶原酶分散的完整大鼠睾丸和睾丸间质细胞产生5 α-雄甾烷-3 α，17 β-二醇（雄甾烷）、雄酮和睾酮。促黄体激素刺激生产的每一个雄激素的细胞制备31至34天的大鼠。每种雄激素产生的半数最大刺激发生在约3.5 ng NIH-LH-B 9/ml培养基中。雄甾烷酮是主要的产物，然后是雄酮，然后是睾酮。促黄体生成素刺激睾丸酮的产生，但不是雄甾烷或雄酮分散间质细胞从200天的大鼠。生产的时间过程和细胞浓度对这些雄激素生产的影响表明，在未成熟动物的分散睾丸间质细胞中，雄甾烷酮和雄甾酮至少部分通过睾酮的代谢形成。在这些实验中LH刺激的睾酮生产增加孵育15- 60分钟，然后保持不变，直到180分钟。在孵育60- 180分钟，雄甾烷酮和雄酮的浓度以线性方式增加。不同的细胞孵育产生的细胞浓度和5 α-还原雄激素：睾酮产生的比率之间的正相关性。促黄体生成激素刺激生产的每一个雄激素的整体测试获得大鼠在30- 175天的年龄。这些大鼠的血清睾酮浓度在50日龄时突然增加。在这个年龄还观察到体外雄激素产生的显著变化包括：（1）在存在或不存在LH的情况下孵育时，三种类固醇的产生增加;（2）在存在或不存在LH的情况下，睾酮的产生增加，这代表了三种雄激素总产生的更大百分比。
The production of 5 alpha-androstane-3 alpha, 17 beta-diol (androstanediol), androsterone and testosterone by whole rat testes and testicular interstitial cells dispersed with collagenase was studied in vitro. Luteinizing hormone stimulated the production of each of the androgens by cells prepared from 31- to 34-day-old rats. Half maximum stimulation of the production of each androgen occurred with approximately 3.5 ng NIH-LH-B9/ml medium. Androstanediol was the predominant product then androsterone and then testosterone. Luteinizing hormone stimulated the production of testerone, but not androstanediol or androsterone by dispersed interstitial cells from 200-day-old rats. The time-course of production and the effect of the concentration of cells on the production of these androgens suggested that in dispersed testicular interstitial cells from immature animals androstanediol and androsterone are formed, at least partially, by the metabolism of testosterone. In these experiments LH-stimulated testosterone production increased during incubation for 15--60 min and then remained constant up to 180 min. The concentrations of androstanediol and androsterone increased in a linear manner during incubation for 60--180 min. Varying the number of cells incubated yielded a positive correlation between cell concentration and the ratio 5 alpha-reduced androgen : testosterone produced. Luteinizing hormone stimulated production of each androgen by whole tests obtained from rats at 30--175 days of age. The serum concentration of testosterone in these rats increased abruptly at 50 days of age. Significant changes in androgen production in vitro also observed at this age included: (1) increased production of the three steroids when incubated in either the presence or absence of LH and (2) testosterone production, either in the presence or absence of LH, which represented a greater percentage of the total production of the three androgens.