General concept of high-performance amperometric detector for microfluidic (bio)analytical chips
General concept of high-performance amperometric detector for microfluidic (bio)analytical chips
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DOI：
10.1021/ac800227t
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发表时间：
2008-07-01
影响因子：
7.4
通讯作者：
Thouin, Laurent
中科院分区：
文献类型：
--
作者：
Amatore, Christian;Da Mota, Nicolas;Thouin, Laurent
在这项工作中，我们从理论上证明，安培检测器阵列由一系列放置在微通道壁上的平行带状微电极组成，在分离阶段后应用到微流控(生物)分析装置上，可以提供良好的分析检测性能。与先前工作中报告的浓度印迹策略相结合，这些特殊的性能可能扩展到非电活性或扩散较差的分析物。使用电极阵列而不是大的单频带，可以探测通道的整个核心，同时保持良好的时间分辨率。因此，保留时间接近的分析物可以单独表征，其分辨率最终超过光谱检测的分辨率。只有完全了解电化学检测器附近的微流体溶液中分子的扩散和对流传输之间的复杂耦合，才能获得这些重要的优势。因此，这项工作中提出的理论数据所依据的条件是在基于先前的理论分析的基础上优化程序后选择的。它们将在一系列进一步的工作中得到充分披露，这些工作还将建立这种安培检测器的实验性能，并验证本概念。
In this work, we established theoretically that amperometric detector arrays consisting of a series of parallel band microelectrodes placed on the wall of a microchannel may offer excellent analytical detection performances when implemented onto microfluidic (bio)analytical devices after the separative stages. In combination with the concentration imprinting strategies reported in a previous work, these exceptional performances may be extended to nonelectroactive or poorly diffusing analytes. Using an array of electrodes instead of a large single band allows the whole core of the channel to be probed though keeping an excellent time resolution. Thus, analytes with close retention times may be characterized individually with a resolution which eventually outpaces that of spectroscopic detections. Such important advantages may be obtained only through a complete understanding of the complex coupling between diffusional and convective transport of molecules in microfluidic solutions near an electrochemical detector. As a consequence, the conditions underlying the theoretical data presented in this work have been selected after optimizing procedures rooted on previous theoretical analyses. They will be fully disclosed in a series of further works that will also establish the experimental performances of such amperometric detectors and validate the present concept.