Effect of pregnancy-specific beta 1-glycoprotein on the development of preimplantation embryo.
Effect of pregnancy-specific beta 1-glycoprotein on the development of preimplantation embryo.
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妊娠特异性β1-糖蛋白对植入前胚胎发育的影响。
DOI：
10.1046/j.1525-1373.1999.d01-25.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Chan,WY
中科院分区：
文献类型：
--
作者：
Wu,SM;Arnold,LL;Rone,J;Trivadi,M;Chan,WY
本研究的目的是检验妊娠特异性 β1-糖蛋白 (PSG) 家族成员促进胚胎生长和成熟的假设。编码 PSG 家族两个成员 PSG1 和 PSG3 的 cDNA 在中国仓鼠卵巢 (CHO) 细胞中用表达载体 pHβAPr-1-neo 表达。在存在和不存在中和 PSG 抗体的情况下，将两细胞阶段小鼠胚胎与表达重组 PSG1 (rPSG1) 或 PSG3 (rPSG3) 的 CHO 细胞在两室系统中共培养。以 12 小时为间隔评估胚胎的卵裂和成熟阶段。与对照相比，与表达rPSG1的转染子共培养的小鼠胚胎显示出裂解和成熟率显着提高，P<0.005-0.004。在与表达 rPSG3 的 CHO 细胞共培养中，在发育早期直至囊胚形成晚期，未观察到与对照细胞的显着差异。在该阶段，与对照组相比，rPSG3 的发育具有统计显着性增强，P < 0.001。这些结果表明 PSG1 和 PSG3 在小鼠模型的不同发育阶段表现出亲胚胎活性。
The objective of this study is to test the hypothesis that members of the pregnancy-specific β1-glycoprotein (PSG) family enhance the growth and maturation of embryos. cDNA encoding two members of the PSG family, namely PSG1 and PSG3, were expressed in Chinese Hamster Ovary (CHO) cells with the expression vector pHβAPr-1-neo. Two-cell stage mouse embryos were co-cultured in a two-chamber system with CHO cells expressing either recombinant PSG1 (rPSG1) or PSG3 (rPSG3) in the presence and absence of neutralizing PSG antibodies. The cleavage and maturation stage of the embryos was assessed at 12-hr intervals. Mouse embryos cocultured with transfectants expressing rPSG1 showed a significant enhancement of cleavage and maturation rate compared to controls withP< 0.005-0.004. In cocultures with CHO cells expressing rPSG3, no significant difference from the controls was observed in the early stage of development until late blastocyst formation. At that stage, there was a statistically significant enhancement of development by rPSG3 when compared to controls withP< 0.001. These results suggest that PSG1 and PSG3 exhibit embryotropic activity at different stages of development in the mouse model.