GROWTH-FACTORS ATTENUATE THE CHOLINOTOXIC EFFECTS OF ETHANOL DURING EARLY NEUROEMBRYOGENESIS IN THE CHICK-EMBRYO
GROWTH-FACTORS ATTENUATE THE CHOLINOTOXIC EFFECTS OF ETHANOL DURING EARLY NEUROEMBRYOGENESIS IN THE CHICK-EMBRYO
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DOI：
10.1016/0736-5748(91)90041-j
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发表时间：
1991-01-01
影响因子：
1.8
通讯作者：
VERNADAKIS, A
中科院分区：
文献类型：
--
作者：
BRODIE, C;KENTROTI, S;VERNADAKIS, A
以胆碱乙酰转移酶活性为胆碱能标记物，研究了生长因子和乙醇对鸡胚胎早期胆碱能神经元表达的影响。正如我们之前所报道的(Brodie&Vernadakis，Dev.脑研究56：223-228,1990；Ktroti和Vernadakis，Dev.Brain Res.56：205-210,1990)，胚胎1-3天在卵子中注射乙醇可使胆碱乙酰转移酶活性降低30%。在胚胎第1~3天单独应用神经生长因子和表皮生长因子，在胚胎第8天检查时，脑和脊髓的胆碱乙酰转移酶活性均略有增加。神经生长因子或表皮生长因子与乙醇联合应用可消除乙醇引起的胆碱乙酰转移酶活性的下降。此外，在胚胎第4-7天给予神经生长因子或表皮生长因子，在第1-3天用乙醇处理的胚胎将胆碱乙酰转移酶活性提高到与对照组相似的水平。因此，生长因子逆转了乙醇诱导的胆碱能损伤，并使胆碱能群体恢复到正常水平。这些发现为NGF和EGF在干预乙醇对胚胎发育的神经毒性作用中可能发挥的作用提供了证据。
The interaction between growth factors and ethanol on cholinergic neuronal expression was studied in the chick embryo during early neuroembryogenesis using choline acetyltransferase activity as a cholinergic marker. As we have previously reported (Brodie & Vernadakis, Dev. Brain Res. 56: 223-228, 1990; Kentroti and Vernadakis, Dev. Brain Res. 56: 205-210, 1990), ethanol administration in ovo at embryonic days 1-3 produced a 30% decrease in choline acetyltransferase activity. Nerve growth factor and epidermal growth factor administration alone, at embryonic days 1-3, produced a slight increase in choline acetyltransferase activity of both brain and spinal cord when examined at embryonic day 8. Concomitant administration of either nerve growth factor or epidermal growth factor with ethanol eliminated the decrease in choline acetyltransferase activity produced by ethanol. Moreover, administration of either nerve growth factor or epidermal growth factor at embryonic days 4-7 to embryos pretreated with ethanol at days 1-3 raised choline acetyltransferase activity to a level similar to that observed in controls. Thus the growth factors reversed the ethanol-induced cholinergic insult and restored the cholinergic population to normal.These findings provide evidence of a possible role of NGF and EGF in interfering with the neurotoxic effects of ethanol during embryogenesis.