TOXOPLASMA-GONDII IN IOWA SOWS - COMPARISON OF ANTIBODY-TITERS TO ISOLATION OF TOXOPLASMA-GONDII BY BIOASSAYS IN MICE AND CATS
TOXOPLASMA-GONDII IN IOWA SOWS - COMPARISON OF ANTIBODY-TITERS TO ISOLATION OF TOXOPLASMA-GONDII BY BIOASSAYS IN MICE AND CATS
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DOI：
10.2307/3284004
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发表时间：
1995-02-01
影响因子：
1.3
通讯作者：
POWELL, EC
中科院分区：
文献类型：
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作者：
DUBEY, JP;THULLIEZ, P;POWELL, EC
对在爱荷华州屠宰场宰杀的1,000头猪的心脏进行了弓形虫组织包囊流行率的生物测定。将来自每头猪的100克心肌均质化，在胃蛋白酶溶液中消化，并在10只小鼠中进行生物测定。还在猫中对来自183头猪的子集中的每头猪的500 g心脏组织进行了生物测定。从每头猪的心脏收集的血清进行抗T测定。弓形虫抗体的改良凝集试验使用福尔马林固定的整个速殖子。抗T弓形虫抗体阳性率为22.2%。维伯河从总共170头猪中分离出弓形虫;通过生物测定从小鼠的50个心脏中分离出弓形虫;通过生物测定从小鼠和猫的58个心脏中分离出弓形虫;通过生物测定仅从猫的62头猪中分离出弓形虫。猫的分离成功率（65.6%）约为小鼠（31.7%）的两倍。T.猪弓形虫抗体滴度倒数（括号内）为：3.7%（
Hearts of 1,000 pigs killed at an abattoir in Iowa were bioassayed for the prevalence of tissue cysts of Toxoplasma gondii. One hundred grams of cardiac muscle from each pig was homogenized, digested in pepsin solution, and bioassayed in 10 mice. Five hundred grams of heart tissue from each of a subset of 183 pigs was also bioassayed in cats. Serum collected from the heart from each pig was assayed for anti-T. gondii antibodies in the modified agglutination test using formalin-fixed whole tachyzoites. Anti-T. gondii antibodies were found in 22.2% of pigs. Viable T. gondii was isolated from a total of 170 pigs; from 50 hearts by bioassay in mice, from 58 hearts by bioassay in both mice and cats, and from 62 pigs by bioassay in cats only. The success of isolation in cats (65.6%) was approximately twice that in mice (31.7%). Percentage of isolations of T. gondii with respect to reciprocal antibody liters (in parentheses) in pigs was: 3.7% (