Neural precursor cells division and migration in neonatal rat brain after ischemic/hypoxic injury
Neural precursor cells division and migration in neonatal rat brain after ischemic/hypoxic injury
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DOI：
10.1016/j.brainres.2004.12.048
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发表时间：
2005-03-15
期刊：
影响因子：
2.9
通讯作者：
Abe, K
中科院分区：
文献类型：
--
作者：
Hayashi, T;Iwai, M;Abe, K
缺血/缺氧（I/H）会导致严重的围产期脑部疾病，例如脑瘫。新生儿大脑具有很强的可塑性，增强新细胞的产生将是治疗此类疾病的创新手段。为了阐明缺血后新生儿大脑中神经祖细胞的动态变化，我们研究了脑室下区新细胞的产生以及这些细胞随后迁移到受损区域的情况。通过掺入溴脱氧尿苷 (BrdU) 来确认新产生的细胞，并通过免疫组织化学研究每个细胞谱系的分子标记来研究分化尝试。在假手术对照组的大脑中，有许多BrdU标记的细胞，随着动物年龄的增长，这些细胞逐渐减少。这些细胞中有许多是少突胶质祖细胞或小胶质细胞。虽然在假对照中只有很少的神经元细胞被标记为BrdU，但在I/H后它们显着增加。它们位于祖细胞所在的室下区正下方和损伤区域，表明新产生的细胞迁移到梗塞区域并分化为神经元前体细胞，以补偿丢失的神经细胞。我们发现 I/H 后，BrdU 标记的星形胶质细胞、少突胶质细胞祖细胞和小胶质细胞也有所增加，表明它们在恢复中也发挥着积极作用。祖细胞可能具有治疗围产期脑部疾病的潜力。 (c) 2005 Elsevier B.V. 保留所有权利。
Ischemia/hypoxia (I/H) causes severe perinatal brain disorders such as cerebral palsy. The neonatal brain possesses much plasticity, and to enhance new cell production would be an innovative means of therapy for such disorders. In order to elucidate the dynamic changes of neural progenitor cells in the neonatal brain after ischemia, we investigated new cells production in the subventricular zone and subsequent migration of these cells to the injured area. Newly produced cells were confirmed by incorporation of bromodeoxyuridine (BrdU), and attempt for differentiation was investigated by immunohistochemistry for molecular markers of each cellular lineage. In the sham-control brain, there were many BrdU-labeled cells which gradually decreased as the animal becomes older. Many of these cells were oligodendroglial progenitor or microglial cells. Although there were only few neuronal cells labeled for BrdU in the sham-control, they dramatically increased after I/H They were located at just beneath the subventricular zone where the progenitor cells reside and to the injured area, indicating that newly produced cells migrated to the infarct region and differentiated into neuronal precursor cells in order to compensate the lost neural cells. We found that BrdU-labeled astroglial, oligodendroglial progenitor, and microglial cells were also increased after I/H, suggesting that they also play active roles in recovery. Progenitor cells may have potential for treating perinatal brain disorders. (c) 2005 Elsevier B.V. All rights reserved.