Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast
Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast
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DOI：
10.1091/mbc.e23-05-0164
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发表时间：
2023-11-01
影响因子：
3.3
通讯作者：
Prosser，Derek C.
中科院分区：
文献类型：
--
作者：
Woodard，Thaddeus K.;Rioux，Daniel J.;Prosser，Derek C.
大多数真核细胞利用网格蛋白介导的内吞作用以及多个网格蛋白独立途径来内化蛋白质和膜。尽管网格蛋白介导的内吞作用已被广泛研究，并且许多机械蛋白已被鉴定，但通过比较，网格蛋白独立途径仍然很少被表征。我们之前确定了第一个已知的酵母网格蛋白独立内吞途径，该途径依赖于肌动蛋白调节 GTPase Rho1、formin Bni1 和无分支肌动蛋白丝，但不需要网格蛋白外壳或核心网格蛋白机械蛋白。在这项研究中，我们试图通过探索肌球蛋白作为基于肌动蛋白的马达的作用来更好地了解酵母中不依赖网格蛋白的内吞作用，因为肌动蛋白是酵母内吞作用所必需的。我们发现Myo2沿着肌动蛋白电缆将分泌囊泡、细胞器和微管运输到极化生长位点，参与不依赖于网格蛋白的内吞作用。出乎意料的是，Myo2 将微管正端转运至细胞皮层的能力似乎是其在不依赖于网格蛋白的内吞作用中发挥作用所必需的。此外，还需要动力蛋白、动力蛋白和参与皮质微管捕获的蛋白质。因此，我们的结果表明肌动蛋白和微管之间的相互作用有助于酵母中不依赖于网格蛋白的内化。
Most eukaryotic cells utilize clathrin-mediated endocytosis as well as multiple clathrin-independent pathways to internalize proteins and membranes. Although clathrin-mediated endocytosis has been studied extensively and many machinery proteins have been identified, clathrin-independent pathways remain poorly characterized by comparison. We previously identified the first known yeast clathrin-independent endocytic pathway, which relies on the actin-modulating GTPase Rho1, the formin Bni1 and unbranched actin filaments, but does not require the clathrin coat or core clathrin machinery proteins. In this study, we sought to better understand clathrin-independent endocytosis in yeast by exploring the role of myosins as actin-based motors, because actin is required for endocytosis in yeast. We find that Myo2, which transports secretory vesicles, organelles and microtubules along actin cables to sites of polarized growth, participates in clathrin-independent endocytosis. Unexpectedly, the ability of Myo2 to transport microtubule plus ends to the cell cortex appears to be required for its role in clathrin-independent endocytosis. In addition, dynein, dynactin, and proteins involved in cortical microtubule capture are also required. Thus, our results suggest that interplay between actin and microtubules contributes to clathrin-independent internalization in yeast.