Expression of mosquito miRNAs in entomopathogenic fungus induces pathogen-mediated host RNA interference and increases fungal efficacy.
Expression of mosquito miRNAs in entomopathogenic fungus induces pathogen-mediated host RNA interference and increases fungal efficacy.
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DOI：
10.1016/j.celrep.2022.111527
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发表时间：
2022-10
期刊：
影响因子：
8.8
通讯作者：
Chunlai Cui;Yan Wang;Yifei Li;Peilu Sun;Jinyong Jiang;Hongning Zhou;Jingnan Liu;Sibao Wang
中科院分区：
文献类型：
--
作者：
Chunlai Cui;Yan Wang;Yifei Li;Peilu Sun;Jinyong Jiang;Hongning Zhou;Jingnan Liu;Sibao Wang
杀虫剂耐药性的威胁日益严重，迫切需要开发更多的蚊子控制工具。昆虫病原真菌为化学杀虫剂提供了一种生态友好的替代品。使用真菌杀虫剂的一个限制是它们相对较低的毒力。在这里，我们报告了一种抑制蚊子免疫力和增加真菌毒力的方法。我们设计了球孢白僵菌（Beauveria bassianato）表达Aedesimmunosuppressive microRNAs（miRNAs）来诱导宿主RNA干扰（RNAi）免疫应答。我们发现，工程菌株可以产生并将miRNA递送到宿主细胞中，以在感染期间激活跨界RNAi，并通过靶向多个宿主基因来抑制蚊子免疫力，从而显著增加真菌对埃及伊蚊和大蜡螟的毒力。重要的是，表达宿主miRNAs还显著增加了真菌对耐药蚊子的毒力，为耐药管理创造了潜力。这种基于病原体介导的RNAi（pmRNAi）的方法提供了一种创新的策略，以提高真菌杀虫剂的功效并消除抗性发展的可能性。
The growing threat of insecticide resistance prompts the urgent need to develop additional tools for mosquito control. Entomopathogenic fungi provide an eco-friendly alternative to chemical insecticides. One limitation to the use of mycoinsecticides is their relatively low virulence. Here, we report an approach for suppressing mosquito immunity and increasing fungal virulence. We engineeredBeauveria bassianato expressAedesimmunosuppressive microRNAs (miRNAs) to induce host RNA interference (RNAi) immune responses. We show that engineered strains can produce and deliver the miRNAs into host cells to activate cross-kingdom RNAi during infection and suppress mosquito immunity by targeting multiple host genes, thereby dramatically increasing fungal virulence againstAedes aegyptiandGalleria mellonellalarvae. Importantly, expressing host miRNAs also significantly increases fungal virulence against insecticide-resistant mosquitoes, creating potential for insecticide-resistance management. This pathogen-mediated RNAi (pmRNAi)-based approach provides an innovative strategy to enhance the efficacy of fungal insecticides and eliminate the likelihood of resistance development.