mRNA expression of the Nurr1 and NGFI-B nuclear receptor families following acute and chronic administration of methamphetamine
mRNA expression of the Nurr1 and NGFI-B nuclear receptor families following acute and chronic administration of methamphetamine
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DOI：
10.1016/j.pnpbp.2008.09.021
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发表时间：
2008-12-12
影响因子：
5.6
通讯作者：
Saito, Atsushi
中科院分区：
文献类型：
--
作者：
Akiyama, Kazufumi;Isao, Taketo;Saito, Atsushi
Nur相关1（Nurr 1）和神经生长因子诱导B（NGFI-B）构成核受体超家族的密切相关的亚组。4 mg/kg急性甲基苯丙胺（METH）给药后1 ~ 3 h，Nurr 1 mRNA水平在前边缘（PrL）、初级运动（M1）和初级躯体感觉（SI）皮质以及腹侧被盖区（VTA）显著高于基础水平。预处理与0.5毫克/公斤的SCH 23390防止急性甲硫氨酸诱导的Nurr 1 mRNA水平在这些脑区的增加。4 mg/kg急性METH给药后1 - 3小时，PrL、M1、S1、纹状体和丘脑核核心（AcbC）中NGFI-B mRNA水平显著增加。用0.5 mg/kg MK-801或0.5 mg/kg SCH 23390预处理可防止这些脑区中急性MET诱导的NGFI-B mRNA水平升高。在先前暴露于盐水或METH（4 mg/kg/天）2周的大鼠中，在三周戒断点处，在挑战注射盐水或4 mg/kg METH后3小时测定mRNA水平。生理盐水激发后，长期暴露于METH的组在PrL、S1和VIA中显示出显著更高的Nurr 1 mRNA水平，在PrL、M1、S1、纹状体和AcbC中显示出显著更高的NGFI-B mRNA水平。长期暴露于METH的组未能增加VTA中的Nurr 1 mRNA和AcbC中的NGFI-B mRNA。当用4 mg/kg METH攻击时。这些结果表明，Nurr 1和NGFI-B mRNA发挥不同的作用后，暴露于甲基。(C)2008年爱思唯尔公司All rights reserved.
Nur-related 1 (Nurr1) and nerve growth factor inducible-B (NGFI-B) constitute closely related subgroups of the nuclear receptor superfamily. One to three hours after 4 mg/kg acute methamphetamine (METH) administration, the levels of Nurr1 mRNA were significantly higher in the prelimbic (PrL), primary motor (M1) and primary somatosensory (SI) cortices and ventral tegmental area (VTA), as compared with the basal level. Pretreatment with 0.5 mg/kg of SCH23390 prevented the acute METH-induced increase in Nurr1 mRNA levels in these brain regions. One to three hours after 4-mg/kg acute METH administration, the levels of NGFI-B mRNA increased significantly in the PrL, M1, S1, striatum, and nucleus accumbens core (AcbC). Pretreatment with either 0.5 mg/kg of MK-801 or 0.5 mg/kg of SCH23390 prevented the acute METH-induced increase in NGFI-B mRNA levels in these brain regions. The levels of mRNAs were determined 3 h after a challenge injection of either saline or 4 mg/kg METH at the three-week withdrawal point in rats which had previously been exposed to either saline or METH (4 mg/kg/day) for 2 weeks. After the saline challenge, the group chronically exposed to METH displayed significantly higher levels of Nurr1 mRNA in the PrL, S1 and VIA, and of NGFI-B mRNA in the PrL, M1, S1, striatum and AcbC than did the group chronically treated with saline. The groups chronically exposed to METH failed to increase Nurr1 mRNA in the VTA, and NGFI-B mRNA in the AcbC. when challenged with 4 mg/kg METH. These results suggest that Nurr1 and NGFI-B mRNA play differential roles upon exposure to METH. (C) 2008 Elsevier Inc. All rights reserved.