The use of the S haplotype-specific F-box protein gene, SFB, as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot (Prunus mume)
The use of the S haplotype-specific F-box protein gene, SFB, as a molecular marker for S-haplotypes and self-compatibility in Japanese apricot (Prunus mume)
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DOI：
10.1007/s00122-003-1389-7
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发表时间：
2003-08
影响因子：
5.4
通讯作者：
H. Yamane;K. Ushijima;H. Sassa;R. Tao
中科院分区：
文献类型：
--
作者：
H. Yamane;K. Ushijima;H. Sassa;R. Tao
梅与其它自交不亲和的李属植物一样，也具有以S-RNase为基础的配子体自交不亲和系统。本研究鉴定了日本杏花粉S单倍型的候选基因--F-box蛋白基因（Shaplotype-specific F-box protein gene，SFB），建立了日本杏花粉S单倍型的分子分型系统。利用SFB基因特异性引物，对“南柯”（S1 S7）的克隆Pm-SFB 1和Pm-SFB 7进行5′和3′ RACE（rapidamplification of cDNA ends）扩增。与其他李属物种的SFB一样，Pm-SFB 1和Pm-SFB 7表现出高水平的S单倍型特异性序列多态性，并且它们的表达对花粉具有特异性。用Pm-SFB探针在低严格条件下对11个日本杏品种进行基因组DNA印迹分析，得到了S1-至S8-单倍型和一个自交不亲和的Sf-单倍型的特异性RFLP条带。本文还讨论了SFBA作为日本杏S-单倍型和自交亲和性分子标记的实际应用。
Japanese apricot (Prunus mume) exhibits the S-RNase-based gametophytic self-incompatibility system as do other self-incompatiblePrunusspecies. This report identifies theShaplotype-specific F-box protein gene (SFB), a candidate gene for pollen-S, of Japanese apricot, which leads to the development of a molecular typing system forS-haplotype in this fruit species. Both 5′- and 3′-RACE (rapid amplification of cDNA ends) were performed withSFBgene-specific oligonucleotide primers to clonePm-SFB1andPm-SFB7of 'Nanko (S1S7)'. As in the case ofSFBof otherPrunusspecies,Pm-SFB1andPm-SFB7showed a high level ofS-haplotype-specific sequence polymorphism and their expression was specific to pollen. Genomic DNA-blot analyses of 11 Japanese apricot cultivars with thePm-SFBprobes under low stringency conditions yielded RFLP bands specific to theS1- toS8-haplotypes as well as a self-compatibleSf-haplotype. A practical usage ofSFBas a molecular marker forS-haplotypes and self-compatibility in Japanese apricot is discussed.