Mouse glycosylphosphatidylinositol-specific phospholipase D (Gpld1) characterization.
Mouse glycosylphosphatidylinositol-specific phospholipase D (Gpld1) characterization.
复制标题
小鼠糖基磷脂酰肌醇特异性磷脂酶 D (Gpld1) 表征。
DOI：
10.1007/s003359900851
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Deeg,MA
中科院分区：
文献类型：
--
作者：
LeBoeuf,RC;Caldwell,M;Guo,Y;Metz,C;Davitz,MA;Olson,LK;Deeg,MA
糖基磷脂酰肌醇特异性磷脂酶D （GPI- pld）是一种在循环中发现的110 kda单体蛋白，能够在洗涤剂存在的情况下降解数十种细胞表面蛋白所利用的GPI锚。该蛋白相对丰富（人血清中5-10 μg/ml），但其合成位点、基因结构和整体功能尚不清楚。我们的目的是利用小鼠系统来确定其在脂质转运、病原体控制和糖尿病中的假定作用。我们从胰腺α细胞文库中分离出小鼠GPI-PLD全长cDNA。推导出的氨基酸序列与牛和人GPI-PLD同源性为74%。在小鼠染色体（Chr） 13上有一个单一的结构基因（Gpld1），在9个组织中，肝脏中GPI-PLD mRNA的丰度最高。血清GPI-PLD活性在四种小鼠品系中存在遗传差异，GPI-PLD活性与这些小鼠高密度脂蛋白循环水平之间没有相关性。这是首次报道gpld1的定位和遗传调控。这些信息将有助于我们进一步研究GPI-PLD在正常和病理条件下的表达和功能。
Glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) is an 110-kDa monomeric protein found in the circulation that is capable of degrading the GPI anchor utilized by dozens of cell-surface proteins in the presence of detergent. This protein is relatively abundant (5–10 μg/ml in human serum), yet its sites of synthesis, gene structure, and overall function are unclear. It is our purpose to use the mouse system to determine its putative roles in lipid transport, pathogen control, and diabetes. We have isolated murine full-length cDNA for GPI-PLD from a pancreatic alpha cell library. The deduced amino acid sequence shows 74% homology to bovine and human GPI-PLD. There is a single structural gene (Gpld1) mapping to mouse Chromosome (Chr) 13, and among nine tissues, liver showed the greatest abundance of GPI-PLD mRNA. Genetic differences in serum GPI-PLD activity were seen among four mouse strains, and no correlation was seen between GPI-PLD activity and circulating levels of high density lipoproteins in these mice. This is the first report of map position and genetic regulation forGpld1.This information will enable us to further study the expression and function of GPI-PLD in normal and pathological conditions.