Measurement of Site-Specific 13C Spin-Lattice Relaxation in a Crystalline Protein
Measurement of Site-Specific 13C Spin-Lattice Relaxation in a Crystalline Protein
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DOI：
10.1021/ja102744b
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发表时间：
2010-06-23
影响因子：
15
通讯作者：
Emsley, Lyndon
中科院分区：
文献类型：
--
作者：
Lewandowski, Jozef R.;Sein, Julien;Emsley, Lyndon
我们证明，它是可能的，以记录特定的自旋-晶格弛豫率为大多数的C-13网站在均匀的C-13和N-15标记的固体蛋白质作为一个结果的质子驱动的自旋扩散在样品自旋频率>= 60 kHz的放缓，从而提供了一系列新的实验探针表征分子动力学在固体蛋白质。
We demonstrate that it is possible to record site-specific spin-lattice relaxation rates for the majority of C-13 sites in uniformly C-13 and N-15 labeled solid proteins as a result of the slowing down of proton-driven spin diffusion at sample spinning frequencies >= 60 kHz, thus providing a series of new experimental probes for characterizing molecular dynamics in solid proteins.