Innate lymphoid cells in the upper airways: importance of CD117 and IL-1RI expression
Innate lymphoid cells in the upper airways: importance of CD117 and IL-1RI expression
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DOI：
10.1183/13993003.00742-2018
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发表时间：
2018-12-01
影响因子：
24.3
通讯作者：
Bachert, Claus
中科院分区：
文献类型：
--
作者：
Van Crombruggen, Koen;Taveirne, Sylvie;Bachert, Claus
尽管1型、2型和3型先天淋巴样细胞（分别为ILC1、ILC2和ILC3）正在成为调节组织稳态、重塑和炎症的重要细胞群，但我们的知识绝大多数来自体外和小鼠实验，需要在人类疾病中得到彻底证实。通过流式细胞术评估了慢性鼻窦炎患者新鲜切除的人上呼吸道粘膜中ILC的相对水平。考虑到患者的临床参数和疾病合并症，可分为息肉 (CRSsNP) 和鼻息肉 (CRSwNP)。我们报告，人 ILC2 的 CD117 和 I 型白细胞介素受体 (IL-1RI) 表达状态取决于局部组织环境。只有仅存在于 CRSwNP 中的 CD117(+) IL-1RI(+) ILC2 与人上呼吸道粘膜中的 2 型 T 辅助细胞细胞因子和嗜酸性粒细胞水平具有相互关系。在 CRSsNP 中，主要是 CD117(-)IL-1RI(-) ILC2 增加，尽管 ILC2 水平较高，但在该疾病中产生较低的嗜酸性粒细胞增多。这些数据表明，与体外数据得出的结论相反，天然人 ILC2 群体中的 CD117(-) 和 CD117(+) 部分不是随机现象，并且 IL-1RI 表达在体内并不普遍存在。人类体内的 ILC2，无法通过体外和小鼠实验进行评估。
Although type 1, 2 and 3 innate lymphoid cells (ILC1s, ILC2s and ILC3s, respectively) are emerging as important cell populations regulating tissue homeostasis, remodelling and inflammation, a vast majority of our knowledge stems from in vitro and murine experiments, and requires thorough confirmation in human diseases.Relative levels of ILCs were evaluated by means of flow cytometry in freshly resected human upper airways mucosa of patients with chronic rhinosinusitis without nasal polyps (CRSsNP) and with nasal polyps (CRSwNP), taking into account the patient's clinical parameters and disease comorbidities.We report that the CD117 and interleukin-receptor type I (IL-1RI) expression status of human ILC2s depends on the local tissue environment. Only CD117(+) IL-1RI(+) ILC2s, exclusively present in CRSwNP, possess an interrelationship with type 2 T-helper cell cytokine and eosinophil levels in human upper airway mucosa. In CRSsNP, mainly CD117(-)IL-1RI(-) ILC2s are increased, yielding lower eosinophilia in this disease despite the high levels of ILC2s.These data unveil that the CD117(-) and CD117(+) fractions within the native human ILC2 population are not a random phenomenon, in contrast to what could be concluded from in vitro data, and that the IL-1RI expression is not ubiquitous in ILC2s in vivo in humans, which cannot be assessed via in vitro and murine experiments.