Dopamine transporter mRNA in autopsy studies of chronic cocaine users
Dopamine transporter mRNA in autopsy studies of chronic cocaine users
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DOI：
10.1016/s0169-328x(99)00233-8
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发表时间：
1999-11-10
期刊：
影响因子：
--
通讯作者：
Mash, DC
中科院分区：
文献类型：
--
作者：
Chen, L;Segal, DM;Mash, DC
采用逆转录-聚合酶链反应（RT-PCR）技术研究了慢性可卡因滥用对中脑多巴胺能神经元多巴胺转运体（DAT）mRNA转录调控的影响。与年龄匹配和无药物对照（CTRL）受试者相比，可卡因过量（CO）受试者黑质（SN）中的DAT/亲环素mRNA比率未发生变化。与此相反，激动型可卡因谵妄患者的DAT mRNA水平显著降低（67%，p < 0.05）。这些研究结果表明可卡因滥用者DAT mRNA的稳态含量缺乏可卡因的调节影响。与此相反，DAT基因的表达显着改变可卡因滥用者在激动性谵妄的风险。(C)1999 Elsevier Science B. V.保留所有权利。
The effects of chronic cocaine abuse on transcriptional regulation of human dopamine transporter (DAT) mRNA in midbrain dopaminergic neurons was assessed by reverse transcription-polymerase chain reaction (RT-PCR). DAT/cyclophilin mRNA ratios in the substantia nigra (SN) were unchanged in cocaine overdose (CO) victims as compared to age-matched and drug free control (CTRL) subjects. In contrast, DAT mRNA levels were decreased significantly in agitated cocaine delirium victims (67%, p < 0.05). These findings demonstrate the lack of a regulatory influence by cocaine on the steady state content of DAT mRNA in cocaine abusers. In contrast, DAT gene expression was altered significantly in cocaine abusers at risk for agitated delirium. (C) 1999 Elsevier Science B.V. All rights reserved.