Potential contribution of neural crest cells to dental enamel formation
Potential contribution of neural crest cells to dental enamel formation
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DOI：
10.1016/j.bbrc.2011.10.026
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发表时间：
2011-11-11
影响因子：
3.1
通讯作者：
Mishina, Yuji
中科院分区：
文献类型：
--
作者：
Wang, Shih-Kai;Komatsu, Yoshihiro;Mishina, Yuji
神经嵴细胞（NCC）是一种多能胚胎细胞群，有助于形成包括牙齿在内的各种颅面结构。一般认为牙釉质是外胚层的衍生物，而牙本质-牙髓复合体和周围的支持组织来源于NCC衍生的间充质。这些传统的概念主要来源于对鱼类和两栖动物的一些早期研究。最近，Wnt 1-Cre/R26 R小鼠，一种用于NCC谱系分析的小鼠模型，揭示了NCC对哺乳动物牙齿发育的贡献。然而，不同的NCC特异性转基因小鼠品系之间的表达模式的差异，使得它必须重新审视哺乳动物牙齿发育的细胞谱系。在这里，我们重新评估了NCC谱系在小鼠牙齿发育过程中使用PO-Cre/R26 R小鼠，另一个NCC特异性转基因小鼠系。与传统观念不一致的是，我们观察到NCC对成釉器官发育和釉质形成的潜在贡献。我们还证明了PO-Cre转基因特异性表达于迁移的NCC在后脑区域，其中NCC有助于牙齿，验证其适用性NCC谱系分析。我们意想不到的发现可能会改变对牙齿发育的一般理解，并为牙齿干细胞生物学提供新的见解。(C)2011 Elsevier Inc. All rights reserved.
Neural crest cells (NCCs) are a multipotent embryonic cell population that contributes to the formation of various craniofacial structures including teeth. It has been generally believed that dental enamel is an ectodermal derivative, whereas the dentin-pulp complex and the surrounding supporting tissues originate from NCC-derived mesenchyme. These traditional concepts stem mainly from several early studies of fishes and amphibians. Recently, Wnt1-Cre/R26R mice, a mouse model for NCC lineage analysis, revealed the contribution of NCCs to mammalian tooth development. However, the discrepancy of expression patterns between different NCC-specific transgenic mouse lines makes it compulsory to revisit the cell lineage in mammalian tooth development. Here, we reevaluated the NCC lineage during mouse tooth development by using PO-Cre/R26R mice, another NCC-specific transgenic mouse line. Inconsistent with the traditional concepts, we observed the potential contribution of NCCs to developing enamel organ and enamel formation. We also demonstrated that the PO-Cre transgene was specifically expressed in migrating NCC in the hindbrain region, where NCC contributes to tooth, validating their applicability for NCC lineage analysis. Our unanticipated finding may change the general understanding of tooth development and provide new insights into dental stem cell biology. (C) 2011 Elsevier Inc. All rights reserved.