Proteomics and N-glycoproteomics analysis of an extracellular matrix-based scaffold-human treated dentin matrix
Proteomics and N-glycoproteomics analysis of an extracellular matrix-based scaffold-human treated dentin matrix
复制标题
基于细胞外基质的支架-人处理的牙本质基质的蛋白质组学和 N-糖蛋白质组学分析
DOI：
10.1002/term.2866
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发表时间：
2019-07-01
影响因子：
3.3
通讯作者：
Song, Jinlin
中科院分区：
文献类型：
--
作者：
Li, Jie;Yang, Hefeng;Song, Jinlin
从哺乳动物组织开发的基于细胞外基质（ECM）的生物材料已成功地用于临床前和临床组织工程应用。我们以前曾报道过牙本质为基础的支架，处理牙本质基质（TDM），牙根再生的适用性。然而，TDM蛋白质组成尚未表征。在这里，我们使用鸟枪蛋白质组学策略来分析人类TDM蛋白质组。通过凝集素亲和层析富集N-糖蛋白，并通过质谱鉴定。将人TDM蛋白质组与已发表的人牙本质蛋白质组进行比较，并进行生物信息学分析。在人TDM中，通过质谱法总共鉴定了708种蛋白质，其中208种是具有318个鉴定的糖基化位点的N-糖蛋白。鉴定了胶原蛋白、蛋白聚糖、小整合素结合配体N-连接糖蛋白（SIBLING）和生长因子，如COL 1A 1、双糖链蛋白聚糖、牙本质唾液蛋白和转化生长因子β 1。糖蛋白在“生物过程”基因本体论术语中富集，例如细胞过程、生物调节、对刺激的响应、代谢过程、免疫系统过程和生物粘附。因此，我们对人TDM蛋白质组的全面研究表明，牙本质蛋白质比以前记录的更异质。我们的发现为设计用于牙根再生和理解牙本质形成的新生物材料提供了线索。
Extracellular matrix (ECM)-based biomaterials developed from mammalian tissues have been successfully used in preclinical and clinical tissue engineering applications. We have previously reported about the applicability of dentin-based scaffold, treated dentin matrix (TDM), for tooth root regeneration. However, TDM protein composition has not been characterized. Here, we used a shotgun proteomic strategy to profile human TDM proteome. N-glycoproteins were enriched by lectin affinity chromatography and identified by mass spectrometry. The total human TDM proteome was compared with the previously published human dentin proteome, and bioinformatics analysis were performed accordingly. In total, 708 proteins were identified by mass spectrometry in human TDM, of which 208 were N-glycoproteins with 318 identified glycosylation sites. Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified. Glycoproteins were enriched in "biological processes" Gene Ontology terms such as cellular process, biological regulation, response to stimulus, metabolic process, immune system process, and biological adhesion. Thus, our comprehensive study of the human TDM proteome revealed that dentin proteins are more heterogeneous than previously documented. Our findings provide clues for designing new biomaterials for tooth root regeneration and understanding dentin formation.