A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation.
A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation.
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DOI：
10.1093/nar/gkp668
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发表时间：
2009-11
影响因子：
14.9
通讯作者：
Romby P
中科院分区：
文献类型：
--
作者：
Geissmann T;Chevalier C;Cros MJ;Boisset S;Fechter P;Noirot C;Schrenzel J;François P;Vandenesch F;Gaspin C;Romby P
对金黄色葡萄球菌基因间隔区的生物信息学分析预测了多个调节区。根据这一分析，我们鉴定了11个新的非编码RNA(RSAA-K)，它们在不同的实验条件下在几个金黄色葡萄球菌菌株中表达。它们中的许多都是在增长的指数后期积累起来的。所有的ncRNA都是稳定的，并且它们的表达是不依赖于Hfq的。其中一些基因的转录受选择性Sigma B因子(RSAA、D和F)的调控，而RsaE的表达则依赖于AGRA。其中6个是金黄色葡萄球菌特有的，4个在其他葡萄球菌中保守，RsaE也存在于芽孢杆菌中。转录学和蛋白质组学分析表明，RsaE调节参与多种代谢途径的蛋白质的合成。系统发育分析结合RNA结构探测、RsaE-mRNA碱基配对搜索和足印分析表明，RsaE的保守和未配对的UCCC序列基序与靶mRNAs结合，阻止核糖体起始复合体的形成。这项研究意外地表明，大多数新的ncRNAs都带有保守的C−富含基序，这表明它们是一类通过共同机制靶向mRNA的ncRNAs的成员。
Bioinformatic analysis of the intergenic regions of Staphylococcus aureus predicted multiple regulatory regions. From this analysis, we characterized 11 novel noncoding RNAs (RsaA‐K) that are expressed in several S. aureus strains under different experimental conditions. Many of them accumulate in the late-exponential phase of growth. All ncRNAs are stable and their expression is Hfq-independent. The transcription of several of them is regulated by the alternative sigma B factor (RsaA, D and F) while the expression of RsaE is agrA-dependent. Six of these ncRNAs are specific to S. aureus, four are conserved in other Staphylococci, and RsaE is also present in Bacillaceae. Transcriptomic and proteomic analysis indicated that RsaE regulates the synthesis of proteins involved in various metabolic pathways. Phylogenetic analysis combined with RNA structure probing, searches for RsaE‐mRNA base pairing, and toeprinting assays indicate that a conserved and unpaired UCCC sequence motif of RsaE binds to target mRNAs and prevents the formation of the ribosomal initiation complex. This study unexpectedly shows that most of the novel ncRNAs carry the conserved C−rich motif, suggesting that they are members of a class of ncRNAs that target mRNAs by a shared mechanism.
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影响因子：
4.8
作者：
Deikus, Gintaras;Bechhofer, David H.
通讯作者：
Bechhofer, David H.
枯草芽孢杆菌和相关物种中转录终止剂的预测。
影响因子：
4.3
作者：
de Hoon, Michiel J L;Makita, Yuko;Nakai, Kenta;Miyano, Satoru
通讯作者：
Miyano, Satoru
影响因子：
3.1
作者：
Cramton, SE;Schnell, NF;Brückner, R
通讯作者：
Brückner, R
DOI：
10.1073/pnas.89.21.10410
发表时间：
1992-11-01
影响因子：
11.1
作者：
AYMERICH, S;STEINMETZ, M
通讯作者：
STEINMETZ, M
影响因子：
10.5
作者：
Boisset, Sandrine;Geissmann, Thomas;Romby, Pascale
通讯作者：
Romby, Pascale