Effect of acute ethanol administration upon hepatic regeneration.
Effect of acute ethanol administration upon hepatic regeneration.
复制标题
急性乙醇给药对肝再生的影响。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
P. Holt
中科院分区：
文献类型：
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作者：
W. Frank;A. Rayyes;A. Washington;P. Holt
摘要本文研究了乙醇对大鼠肝实质细胞再生的影响，其中68%的肝切除刺激了肝再生。乙醇，2.67克/公斤，每日3次，抑制3 H-TdR掺入肝DNA 18至48小时后，部分肝切除术。切除后24小时，对照组动物的峰值3 H-DNA比活性被乙醇给药抑制90%以上。在DNA合成的准备阶段，乙醇损害3 H-DNA标记。当吡唑抑制肝脏乙醇代谢时，3 H-DNA标记仍被抑制。另一方面，肝脏重量显着增加乙醇喂养大鼠切除后72至96小时，乙醇后的总肝DNA含量不低于对照组。乙醇给药加重了再生过程中发生的实质TG蓄积，并增加了肝总蛋白含量。它的结论是，急性乙醇管理不损害部分切除大鼠肝脏的再生反应。抑制3 H-TdR掺入肝DNA在乙醇喂养过程中，不是由于在肝内的浓度和分布的标记胸腺嘧啶核苷的变化，也没有增加胸腺嘧啶的代谢。使用部分肝切除大鼠模型研究药物对肝再生的影响，除了测定3 H-TdR掺入肝DNA外，还应包括测定肝DNA和其他组分的含量。
Abstract The effect of ethanol administration upon hepatic parenchymal cell regeneration was studied in rats in which regeneration of the liver was stimulated by a 68% hepatectomy. Ethanol, 2.67 gm/kg in 3 daily doses, inhibited 3 H-TdR incorporation into hepatic DNA 18 to 48 hr following partial hepatic resection. Peak 3 H-DNA specific activity in control animals 24 hr after resection was inhibited over 90% by ethanol administration. Ethanol impaired 3 H-DNA labeling when given in the preparative phase for DNA synthesis. When hepatic ethanol metabolism was suppressed by pyrazole, 3 H-DNA labeling was still inhibited. On the other hand, hepatic weight was significantly increased in ethanol-fed rats 72 to 96 hr after resection, and total hepatic DNA content after ethanol was no less than that of controls. Ethanol administration accentuated the parenchymal TG accumulation that occurs during regeneration and increased total hepatic protein content. It is concluded that acute ethanol administration does not impair the regenerative response of the partially resected rat liver. Suppression of 3 H-TdR incorporation into hepatic DNA during ethanol feeding was not due to changes in intrahepatic concentration and distribution of the labeled thymidine nor to increased metabolism of thymine. Studies of the effect of agents upon liver regeneration, using the partial hepatectomy rat model, should include measurement of the hepatic content of DNA and other components in addition to the determination of 3 H-TdR incorporation into hepatic DNA.