Immobilization of functional light antenna structures derived from the filamentous green bacterium Chloroflexus aurantiacus.
Immobilization of functional light antenna structures derived from the filamentous green bacterium Chloroflexus aurantiacus.
复制标题
来自丝状绿色细菌橙色绿屈菌的功能性光天线结构的固定化。
作者：
Arati Sridharan;J. Muthuswamy;J. LaBelle;V. Pizziconi
将高效的天然光合光天线结构集成到工程系统中，同时保持其生物光子能力，这在生物混合光子器件的设计中一直是一个难以捉摸的目标。在这项研究中，我们报告了一种新的技术，共价纳米尺度的细菌光天线结构，称为chlorosomes从Chloroflexus aurantiacus在导电和非导电玻璃，同时保持其能量转换功能。无反应中心的绿小体用戊二醛连接体共价固定在3-氨基丙基三乙氧基硅烷（APTES）处理过的表面上。原子力显微镜技术证实，绿色体保持其天然的椭圆形的超微结构固定后。从吸光度和荧光光谱分析（其中斯托克斯位移到808/810 nm时，观察到470 nm的蓝光激发）结合共聚焦显微镜的结果证实，固定化的叶绿体的功能完整性也得到了保护。此外，电化学阻抗谱（EIS）的实验表明，在电极的双电层中的绿小体的存在下，提高了电化学电池的电子转移容量。此外，计时电流研究表明，在叶绿体内发现的Bchl-c色素的还原形式调节电化学电池的传导性质，其中Bchl-c色素的氧化形式阻碍电化学电池内0.4V偏压下的任何电流转导.因此，结果表明，完整的绿小体可以成功地固定化，而它们的生物光子转导能力通过固定化过程中得到保留。这些研究结果表明，它是可行的，设计生物光子器件纳入全功能的光天线结构，这可能会提供显着的性能增强目前的硅基光子器件的各种技术应用，从CCD设备用于视网膜植入地面和空间燃料电池的应用。
The integration of highly efficient, natural photosynthetic light antenna structures into engineered systems while their biophotonic capabilities are maintained has been an elusive goal in the design of biohybrid photonic devices. In this study, we report a novel technique to covalently immobilize nanoscaled bacterial light antenna structures known as chlorosomes from Chloroflexus aurantiacus on both conductive and nonconductive glass while their energy transducing functionality was maintained. Chlorosomes without their reaction centers (RCs) were covalently immobilized on 3-aminoproyltriethoxysilane (APTES) treated surfaces using a glutaraldehyde linker. AFM techniques verified that the chlorosomes maintained their native ellipsoidal ultrastructure upon immobilization. Results from absorbance and fluorescence spectral analysis (where the Stokes shift to 808/810 nm was observed upon 470 nm blue light excitation) in conjunction with confocal microscopy confirm that the functional integrity of immobilized chlorosomes was also preserved. In addition, experiments with electrochemical impedance spectroscopy (EIS) suggested that the presence of chlorosomes in the electrical double layer of the electrode enhanced the electron transfer capacity of the electrochemical cell. Further, chronoamperometric studies suggested that the reduced form of the Bchl- c pigments found within the chlorosome modulate the conduction properties of the electrochemical cell, where the oxidized form of Bchl- c pigments impeded any current transduction at a bias of 0.4 V within the electrochemical cell. The results therefore demonstrate that the intact chlorosomes can be successfully immobilized while their biophotonic transduction capabilities are preserved through the immobilization process. These findings indicate that it is feasible to design biophotonic devices incorporating fully functional light antenna structures, which may offer significant performance enhancements to current silicon-based photonic devices for diverse technological applications ranging from CCD devices used in retinal implants to terrestrial and space fuel cell applications.