In vitro recall of proliferative and cytolytic responses to minor histocompatibility antigens by dendritic cell enriched canine peripheral blood mononuclear cells.
In vitro recall of proliferative and cytolytic responses to minor histocompatibility antigens by dendritic cell enriched canine peripheral blood mononuclear cells.
复制标题
体外回忆富含树突状细胞的犬外周血单核细胞对次要组织相容性抗原的增殖和细胞溶解反应。
DOI：
10.1097/00007890-199501150-00020
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发表时间：
1995
期刊：
影响因子：
6.2
通讯作者：
Bean,MA
中科院分区：
文献类型：
--
作者：
Kalhs,P;White,JS;Gervassi,A;Storb,R;Bean,MA
很难在体外证明犬和人对次要组织相容性抗原存在体内致敏性。使用传统的单向混合白细胞培养，当致敏的血细胞与MHC抗原匹配的同胞PBMC携带次要组织相容性抗原刺激时，通常没有检测到增殖或细胞毒性反应。我们以前报道过，0的17只狗致敏的狗输注犬白细胞抗原相同的同窝血有增殖反应的混合白细胞培养时，未分级的同胞PBMC作为刺激细胞。我们推断，这一结果可能是由于未分级的PBMC不能有效地将次要组织相容性抗原呈递给体内致敏的T细胞，这一功能被认为最好由树突状细胞执行。当我们使用犬PBMC的低浮力密度Percoll组分（先前显示富含树突状细胞）作为刺激细胞时，我们能够在所有5只犬的混合白细胞培养物中产生细胞毒性和/或增殖反应，这些犬通过输注犬白细胞抗原相同的同胞同窝出生仔血液对次要组织相容性抗原致敏。相比之下，使用未分级的PBMC作为刺激细胞，我们仅在5只狗中的1只中发现了致敏证据。这些数据支持的概念，介绍次要组织相容性抗原，主要组织相容性抗原，免疫系统可能仅限于一个亚群的专业APC。
It is difficult in vitro to demonstrate existent in vivo sensitization of dogs and humans to minor histocompatibility antigens. Using conventional one-way mixed leukocyte culture, when sensitized blood cells are stimulated with MHC antigen-matched sibling PBMC bearing the minor histocompatibility antigens, there is usually no proliferative or cytotoxic response detected. We reported previously that 0 of 17 dogs sensitized by transfusion of dog leukocyte antigen-identical littermate blood had proliferative responses in mixed leukocyte culture when unfractionated sibling PBMC were used as stimulator cells. We reasoned that this result might be due to the inability of unfractionated PBMC to efficiently present minor histocompatibility antigens to the in vivo-primed T cells, a function thought best performed by dendritic cells. When we used a low buoyant density Percoll fraction of canine PBMC, shown previously to be enriched in dendritic cells, as stimulator cells, we were able to generate cytotoxic and/or proliferative responses in mixed leukocyte culture in all 5 dogs that had been sensitized to minor histocompatibility antigens by transfusions of dog leukocyte antigen-identical sibling littermate blood. By contrast, using unfractionated PBMC as stimulator cells, we found evidence of sensitization in only 1 of the 5 dogs. These data support the concept that the presentation of minor histocompatibility antigens, in contrast to major histocompatibility antigens, to the immune system may be restricted to a subpopulation of professional APC.