In Vitro Maturation and Fertilization of Immature Oocytes: A Comparative Study of Fertilization Techniques
In Vitro Maturation and Fertilization of Immature Oocytes: A Comparative Study of Fertilization Techniques
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未成熟卵母细胞的体外成熟和受精：受精技术的比较研究
DOI：
10.1023/a:1009450014064
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发表时间：
2004
影响因子：
3.1
通讯作者：
Yieh‐Loong Tsai
中科院分区：
文献类型：
--
作者：
Jiann‐Loung Hwang;Yu;Yieh‐Loong Tsai
摘要目的：探讨影响未成熟卵母细胞成熟和受精的因素。
方法：取剖宫产妇女的未成熟卵母细胞。分别在含或不含卵丘细胞的饱和培养液中培养。成熟到中期II期后，随机行体外受精(IVF)或卵胞浆内精子注射(ICSI)。
结果：培养48h后，441个卵母细胞(42.8%)达到中期II期，其中56.6%的卵丘包被卵母细胞达到中期II期，而裸卵仅29.2%，其中IVF和ICSI受精率分别为56.3%和84.1%(P<0.01)。152枚裸卵中，IVF受精率为39.5%，ICSI受精率为84.5%(P<；0.01)。然而，卵裂率是相似的。
结论：卵丘细胞有利于人卵母细胞的体外成熟，ICSI可提高体外成熟卵母细胞的受精率。然而，无论受精方法或卵丘细胞的存在与否，受精卵母细胞的发育潜力都是相似的。
AbstractPurpose: Our purpose was to investigate the factorsinfluencing maturation and fertilization of immature oocytes.
Methods: Immature oocytes were obtained from womenundergoing cesarean section. They were cultured in thematuration medium either with or without cumulus cells. Aftermaturation to metaphase II, they were randomly fertilizedby in vitro fertilization (IVF) or intracytoplasmic sperminjection (ICSI).
Results: After incubation for 48 hr, 441 oocytes (42.8%)reached metaphase II. Among them, 56.6% ofcumulus-enclosed oocytes, but only 29.2% of denuded oocytes,reached metaphase II. Of the 289 cumulus-enclosed oocytes,the fertilization rates by IVF and ICSI were 56.3 and 84.1%,respectively (P < 0.01). Of the 152 denuded oocytes, thefertilization rates by IVF and ICSI were 39.5 and 84.5%,respectively (P < 0.01). The cleavage rates, however,were similar.
Conclusions: Cumulus cells are beneficial in the maturationof human oocytes in vitro and that ICSI increases thefertilization rate for the in vitro matured oocytes. Thedevelopmental potential of the fertilized oocytes, however, is similarirrespective of the fertilization method or the presence orabsence of cumulus cells.